Abstract
In order to examine underlying mechanisms of the impressive regeneration in Dendrorhynchus Zhejiangensis, a two-dimensional gel electrophoresis (2-DE) combined with mass spectrometric analysis was employed. 70 proteins involved in regenerative process were identified and 33 proteins were up-regulated and others were down-regulated in fracture injured model. Among them, about 79% were cell cytoskeleton proteins and motor proteins. As the representatives, the expression profiles of Actin and Gelsolin-like protein (GSN), both of which were involved in cell motility, in response to body breakage of nemerteans were examined. Two genes showed similar expression patterns during regeneration process and both of them were first constantly up-regulated at 1h and 3h after being broken, then down-regulated to a lower level at 3rd day. The results suggested that Actin and GSN play important roles in regeneration of D. Zhejiangensis.
Keywords: Two-dimensional gel electrophoresis, gelsolin-like protein, actin; nemertean, regeneration, expression.
Graphical Abstract
Current Proteomics
Title:Identification of Differentially Expressed Proteins During Regeneration of Dendrorhynchus zhejiangensis Using the Comparative Proteomic Analysis
Volume: 12 Issue: 3
Author(s): Ye Li, Lei Chen, Jun Zhou, Xiurong Su and Chundan Zhang
Affiliation:
Keywords: Two-dimensional gel electrophoresis, gelsolin-like protein, actin; nemertean, regeneration, expression.
Abstract: In order to examine underlying mechanisms of the impressive regeneration in Dendrorhynchus Zhejiangensis, a two-dimensional gel electrophoresis (2-DE) combined with mass spectrometric analysis was employed. 70 proteins involved in regenerative process were identified and 33 proteins were up-regulated and others were down-regulated in fracture injured model. Among them, about 79% were cell cytoskeleton proteins and motor proteins. As the representatives, the expression profiles of Actin and Gelsolin-like protein (GSN), both of which were involved in cell motility, in response to body breakage of nemerteans were examined. Two genes showed similar expression patterns during regeneration process and both of them were first constantly up-regulated at 1h and 3h after being broken, then down-regulated to a lower level at 3rd day. The results suggested that Actin and GSN play important roles in regeneration of D. Zhejiangensis.
Export Options
About this article
Cite this article as:
Li Ye, Chen Lei, Zhou Jun, Su Xiurong and Zhang Chundan, Identification of Differentially Expressed Proteins During Regeneration of Dendrorhynchus zhejiangensis Using the Comparative Proteomic Analysis, Current Proteomics 2015; 12 (3) . https://dx.doi.org/10.2174/157016461203151120094027
DOI https://dx.doi.org/10.2174/157016461203151120094027 |
Print ISSN 1570-1646 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6247 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Novel Next-Generation Sequencing and Networks-Based Therapeutic Targets: Realistic and More Effective Drug Design and Discovery
Current Pharmaceutical Design Epigenetic Regulation of Cytochrome P450 Enzymes and Clinical Implication
Current Drug Metabolism Gold and Silver Nanoparticles for Applications in Theranostics
Current Topics in Medicinal Chemistry RNA Interference as a Therapeutic Strategy for the Treatment of Liver Diseases
Current Pharmaceutical Design Testicular Germ Cell Tumors: A Paradigm for the Successful Treatment of Solid Tumor Stem Cells
Current Cancer Therapy Reviews Regulatory T Cells and Skin Tumors
Recent Patents on Inflammation & Allergy Drug Discovery Moving to the Rhythm with Clock (Circadian) Genes, Autophagy, mTOR, and SIRT1 in Degenerative Disease and Cancer
Current Neurovascular Research Signaling Processes in Tumoral Neuroendocrine Pituitary Cells as Potential Targets for Therapeutic Drugs
Current Drug Targets - Immune, Endocrine & Metabolic Disorders EphA2-Dependent Molecular Targeting Therapy for Malignant Tumors
Current Cancer Drug Targets The Role of Cytochrome P450 in Herb-Drug Interactions
Current Pharmacogenomics The Dark Side of Stem Cells: Triggering Cancer Progression by Cell Fusion
Current Molecular Medicine Angiotensin II Type 1 Receptor Binding Molecule ATRAP as a Possible Modulator of Renal Sodium Handling and Blood Pressure in Pathophysiology
Current Medicinal Chemistry Role of Protein Tyrosine Phosphatase in Regulation of Cell Signaling Cascades Affecting Tumor Cell Growth: A Future Perspective as Anti-Cancer Drug Target
Current Pharmaceutical Biotechnology The Determination and Use of Optimized Protease Substrates In Drug Discovery and Development
Current Pharmaceutical Design Modulation of Proinflammatory Bacteria- and Lipid-Coupled Intracellular Signaling Pathways in a Transwell Triple Co-Culture Model by Commensal Bifidobacterium Animalis R101-8
Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry Hypoxia-inducible Factor (HIF) in Hormone Signaling During Health and Disease
Cardiovascular & Hematological Agents in Medicinal Chemistry T-Cell Zeta Chain Expression, Phosphorylation and Degradation and their Role in T-Cell Signal Transduction and Immune Response Regulation in Health And Disease
Current Signal Transduction Therapy Bone Invasive Properties of Oral Squamous Cell Carcinoma and its Interactions with Alveolar Bone Cells: An In Vitro Study
Current Cancer Drug Targets Monoclonal Antibody Therapies Targeting Pancreatic Ductal Adenocarcinoma
Current Drug Discovery Technologies Costimulation, Coinhibition and Cancer
Current Cancer Drug Targets