Abstract
In order to examine underlying mechanisms of the impressive regeneration in Dendrorhynchus Zhejiangensis, a two-dimensional gel electrophoresis (2-DE) combined with mass spectrometric analysis was employed. 70 proteins involved in regenerative process were identified and 33 proteins were up-regulated and others were down-regulated in fracture injured model. Among them, about 79% were cell cytoskeleton proteins and motor proteins. As the representatives, the expression profiles of Actin and Gelsolin-like protein (GSN), both of which were involved in cell motility, in response to body breakage of nemerteans were examined. Two genes showed similar expression patterns during regeneration process and both of them were first constantly up-regulated at 1h and 3h after being broken, then down-regulated to a lower level at 3rd day. The results suggested that Actin and GSN play important roles in regeneration of D. Zhejiangensis.
Keywords: Two-dimensional gel electrophoresis, gelsolin-like protein, actin; nemertean, regeneration, expression.
Graphical Abstract
Current Proteomics
Title:Identification of Differentially Expressed Proteins During Regeneration of Dendrorhynchus zhejiangensis Using the Comparative Proteomic Analysis
Volume: 12 Issue: 3
Author(s): Ye Li, Lei Chen, Jun Zhou, Xiurong Su and Chundan Zhang
Affiliation:
Keywords: Two-dimensional gel electrophoresis, gelsolin-like protein, actin; nemertean, regeneration, expression.
Abstract: In order to examine underlying mechanisms of the impressive regeneration in Dendrorhynchus Zhejiangensis, a two-dimensional gel electrophoresis (2-DE) combined with mass spectrometric analysis was employed. 70 proteins involved in regenerative process were identified and 33 proteins were up-regulated and others were down-regulated in fracture injured model. Among them, about 79% were cell cytoskeleton proteins and motor proteins. As the representatives, the expression profiles of Actin and Gelsolin-like protein (GSN), both of which were involved in cell motility, in response to body breakage of nemerteans were examined. Two genes showed similar expression patterns during regeneration process and both of them were first constantly up-regulated at 1h and 3h after being broken, then down-regulated to a lower level at 3rd day. The results suggested that Actin and GSN play important roles in regeneration of D. Zhejiangensis.
Export Options
About this article
Cite this article as:
Li Ye, Chen Lei, Zhou Jun, Su Xiurong and Zhang Chundan, Identification of Differentially Expressed Proteins During Regeneration of Dendrorhynchus zhejiangensis Using the Comparative Proteomic Analysis, Current Proteomics 2015; 12 (3) . https://dx.doi.org/10.2174/157016461203151120094027
DOI https://dx.doi.org/10.2174/157016461203151120094027 |
Print ISSN 1570-1646 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6247 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Nasal Polyposis: An Overview of Differential Diagnosis and Treatment
Recent Patents on Inflammation & Allergy Drug Discovery Circumventing Immune Tolerance Through Epigenetic Modification
Current Pharmaceutical Design The Production and Role of Gastrin-17 and Gastrin-17-Gly in Gastrointestinal Cancers
Protein & Peptide Letters Stem Cell Dynamics and Heterogeneity: Implications for Epidermal Regeneration and Skin Cancer
Current Medicinal Chemistry The ECL Cell: Relay Station for Gastric Integrity
Current Medicinal Chemistry Galectin-9 in Cancer Therapy
Recent Patents on Endocrine, Metabolic & Immune Drug Discovery (Discontinued) CHIP Knockdown Reduced Heat Shock Response and Protein Quality Control Capacity in Lens Epithelial Cells
Current Molecular Medicine The Effect on the Intestine of Some Fungal Toxins: The Trichothecenes
Current Immunology Reviews (Discontinued) The Exploitation of Toll-like Receptor 3 Signaling in Cancer Therapy
Current Pharmaceutical Design Neuronal Acetylcholine Nicotinic Receptors as New Targets for Lung Cancer Treatment
Current Pharmaceutical Design Nanoparticulate Drug Delivery System to Overcome the Limitations of Conventional Curcumin in the Treatment of Various Cancers: A Review
Drug Delivery Letters Bortezomib as the First Proteasome Inhibitor Anticancer Drug: Current Status and Future Perspectives
Current Cancer Drug Targets HR MAS MR Spectroscopy in Metabolic Characterization of Cancer
Current Topics in Medicinal Chemistry Therapeutic Strategies to Target TGF-β in the Treatment of Bone Metastases
Current Pharmaceutical Biotechnology Porphyrins in Photodynamic Therapy - A Search for Ideal Photosensitizers
Current Medicinal Chemistry - Anti-Cancer Agents Use of Lactobacilli and their Pheromone-Based Regulatory Mechanism in Gene Expression and Drug Delivery
Current Pharmaceutical Biotechnology Role of Preoperative Diagnostic Imaging Procedures in Secondary Hyperparathyroidism
Current Medical Imaging Resveratrol, a Phytochemical Inducer of Multiple Cell Death Pathways: Apoptosis, Autophagy and Mitotic Catastrophe
Current Medicinal Chemistry Distinctive Cellular Roles for Novel Protein Kinase C Isoenzymes
Current Pharmaceutical Design TGF-ß / Smad Signaling in Prostate Cancer
Current Drug Targets