Heterogeneous Nuclear Ribonucleoprotein A2/B1 as a Target Antigen in Han Chinese for BD Patients

Title:Heterogeneous Nuclear Ribonucleoprotein A2/B1 as a Target Antigen in Han Chinese for BD Patients

Volume: 22 Issue: 6

Author(s): Jinghui Liang, Weikang Yang, Xiangyu Meng, Peng Chen and Hongwu Du

Affiliation:

Keywords: Autoantigen, Behcet's disease, HnRNP A2/B1, Protein Overexpression and Purification.

Abstract: Behcet's disease (BD) is a recurrent pathema with a typical symptom of inflammation involved in many organs. Previous report indicated that the serum of Korean patients with BD stimulates membrane expression of hnRNP A2/B1 in endothelial cells. In this study, the target 35 kDa recombinant human hnRNP A2/B1 were over-expressed and purified, then sequenced with MALDI-TOF-TOF mass spectrometry. Western blotting and ELISA were applied to detect serum reactivity against hnRNP A2/B1 respectively. The results demonstrate that hnRNP A2/B1 is an autoantigen of BD in Han Chinese population.

Cite this article as:

Liang Jinghui, Yang Weikang, Meng Xiangyu, Chen Peng and Du Hongwu, Heterogeneous Nuclear Ribonucleoprotein A2/B1 as a Target Antigen in Han Chinese for BD Patients, Protein & Peptide Letters 2015; 22 (6) . https://dx.doi.org/10.2174/0929866522666150428121442