Virtual Screening and Discovery of Novel Aurora Kinase Inhibitors

Title:Virtual Screening and Discovery of Novel Aurora Kinase Inhibitors

Volume: 14 Issue: 17

Author(s): R. Raghu, Vinod Devaraji, K. Leena, Sd. Riyaz, Polavaru Baby Rani, Suneel Kumar. B, Pradeep Kumar Naik, P.K. Dubey, Devadasan Velmurugan and M. Vijayalakshmi

Affiliation:

Keywords: Aurora kinase, cancer, docking, glide score, in-vitro cell line studies, PDB.

Abstract: Cancer remains one of the major contributors to human mortality and a hazard to human growth. The search for a new treatment continues unabated. Aurora kinases play an important role in cell cycle, and thus a potential target for the treatment of cancer. In the present work, we aim to discover potential leads against aurora kinase using various rational methods of drug discovery. The available crystal complexes of AKs were analyzed for their interactions and quantified with glide-extra precision (XP) docking. About 20 crystal pdb were selected from the protein databank based on the resolution factor, R-factor and R-value. And after docking with the native ligands, the RMSD value was calculated, wherein the protein with the least RMSD was found to be 3UOK which was further used for our screening of small molecules from the in-house database by molecular docking. Fragments which were found to possess the best interactions were considered for the synthesis with characterization, and biological activity was carried out against breast cancer and colorectal cancer cell lines to assess the inhibitory capability of synthesized compounds. Molecule with the molecular id IS2 i.e. (3E)-3-(5-fluoro-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-2H chromene-2,4(3H)-dione was found to possess inhibitory activity with an IC₅₀ of 1.324nM and 5.785µM for breast cell line and colorectal cell line studies, respectively.

Cite this article as:

Raghu R., Devaraji Vinod, Leena K., Riyaz Sd., Rani Baby Polavaru, B Kumar. Suneel, Naik Kumar Pradeep, Dubey P.K., Velmurugan Devadasan and Vijayalakshmi M., Virtual Screening and Discovery of Novel Aurora Kinase Inhibitors, Current Topics in Medicinal Chemistry 2014; 14 (17) . https://dx.doi.org/10.2174/1568026614666140929151140