SNAP-tag as a Tool for Surface Immobilization

Title:SNAP-tag as a Tool for Surface Immobilization

Volume: 19 Issue: 30

Author(s): Sinem Engin, Dagmar Fichtner, Doris Wedlich and Ljiljana Fruk

Affiliation:

Keywords: SNAP-tag, fusion proteins, benzylguanine, micro-contact printing, protein-nanoparticle conjugation, DNA directed immobilization, DNA origami.

Abstract: SNAP-tag technology has been an important tool for protein study for more than a decade and in the meanwhile has found a number of applications beyond the field of molecular biology and protein purification. Based on covalent interaction of SNAP-tag, 20 kDA mutant of DNA repair protein and benzylguanine, it enables irreversible and controllable protein modification. In this mini review, recent developments in the use of SNAP-tag for the design of protein arrays and nanoparticle biofunctionalization are presented and discussed. A short overview of other applications that paved the way to surface modifications is also given with emphasis on fluorescent labeling through the use of SNAP-tag fusion proteins. Finally, the future of the SNAP-tag methodology for surface patterning and 3D structural scaffolding is addressed.

Cite this article as:

Engin Sinem, Fichtner Dagmar, Wedlich Doris and Fruk Ljiljana, SNAP-tag as a Tool for Surface Immobilization, Current Pharmaceutical Design 2013; 19 (30) . https://dx.doi.org/10.2174/1381612811319300015