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当代肿瘤药物靶点

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ISSN (Print): 1568-0096
ISSN (Online): 1873-5576

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Research Article

口腔鳞状细胞癌的骨侵袭特性及其与牙槽骨细胞的相互作用：体外研究

卷 19, 期 8, 2019

页: [631 - 640] 页: 10

弟呕挨: 10.2174/1568009618666181102144317

价格: $65

摘要

背景：癌细胞与牙槽骨细胞的共培养可以调节骨侵袭和破坏。 然而，口腔鳞状细胞癌（OSCC）与骨细胞之间相互作用的机制仍不清楚。 目的：本研究旨在分析OSCC细胞刺激溶骨活动和骨侵袭的直接和间接影响。 方法：通过用初级牙槽骨细胞系培养OSCC（TCA8113）实现直接共培养。 在间接共培养中，收集TCA8113细胞的上清液以培养牙槽骨细胞。 为了评估骨侵袭特性，进行了体外测定。 结果：与单层对照细胞相比，共培养的癌细胞的增殖显着（p <0.05）更高。 然而，直接和间接共培养细胞的增殖率没有显着差异，间接共培养细胞比直接共培养细胞增殖略多。 与对照单层对应物相比，共培养的OSCC和牙槽骨细胞的侵袭和迁移能力显着增强（p <0.05）。 最重要的是，我们注意到直接与牙槽骨细胞共培养的OSCC细胞刺激了明显的骨胶原破坏。 此外，干细胞和上皮细胞间质转化标志物在共培养细胞中的表达显示出显着变化。 结论：总之，本研究的结果突出了牙槽骨细胞和OSCC细胞在骨侵袭发病机制中共培养环境中相互作用的重要性。 这可能有助于开发未来潜在的OSCC骨侵袭生物疗法。

关键词: 口腔鳞状细胞癌，骨侵袭，共培养，牙槽骨细胞，干细胞标志物，EMT标志物。

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