Production of 68Ga-citrate Based on a SnO2 Generator for Short-Term Turpentine Oil-Induced Inflammation Imaging in Rats

Title:Production of 68Ga-citrate Based on a SnO2 Generator for Short-Term Turpentine Oil-Induced Inflammation Imaging in Rats

Volume: 9 Issue: 3

Author(s): Alireza Mirzaei, Amir R. Jalilian, Mehdi Akhlaghi and Davood Beiki

Affiliation:

Keywords: ⁶⁸Ga-citrate, quality control, turpentine oil, PET/CT, rats, nuclids.

Abstract: Introduction: Gallium-68 citrate has been successfully applied in the PET imaging of infections and inflammation in some centers; however further evaluation of the tracer in inflammation models is of great importance.

Methods: ⁶⁸Ga-citrate prepared from [⁶⁸Ga]GaCl₃ (eluted form an SnO₂ based ⁶⁸Ge/⁶⁸Ga generator) and sodium citrate at optimized conditions followed by quality control tests was injected to normal and turpentine-oil induced rats PET/CT imaging studies up to 290 min.

Results: ⁶⁸Ga-citrate was prepared with acceptable radiochemical purity (>99 ITLC, >99% HPLC), specific activity (28-30 GBq/mM), chemical purity (Sn, Fe <0.3 ppm; Zn<0.2 ppm) in 15 min at 50°C. PET/CT imaging of the tracer demonstrated early detection of inflamed site in animal models in 60-80 min.

Conclusion: This study demonstrated possible early detection of inflammation foci in vivo using ⁶⁸Ga-citrate prepared using commercially available ⁶⁸Ge/⁶⁸Ga generators for PET imaging.

Cite this article as:

Mirzaei Alireza, Jalilian R. Amir, Akhlaghi Mehdi and Beiki Davood, Production of 68Ga-citrate Based on a SnO2 Generator for Short-Term Turpentine Oil-Induced Inflammation Imaging in Rats, Current Radiopharmaceuticals 2016; 9 (3) . https://dx.doi.org/10.2174/1874471009666160506130108