Abstract
Aerva lanata is a prostrate under-shrub used as traditional and modern system of medicine. The Prime of current study was to compare the antiproliferative and antioxidant potential of in vitro and in vivo leaf and callus extracts of Aerva lanata against human breast cancer (MCF 7) cell lines. Anticancer proficiency of methanol extracts of in vitro and in vivo leaf and matured callus extracts were evaluated by 3 - (4, 5 – dimethylthiazole – 2 – yl) - 2, 5 -diphenyl tetrazolium bromide (MTT) and DNA fragmentation assays. Earlier the total antioxidant potential of the different solvent extracts was tested by 2, 2' – azino – bis (3-ethylbenzthiazoline – 6 – sulphonic acid (ABTS) assay. A dose dependent inhibition of cancer cell proliferation was identified in MTT assay. Further, DNA fragmentation assay was performed and the results show the best IC50 values produced best possible digestion of DNA (by apoptosis). The results of radical scavenging activity of ABTS cation assay was recorded promising inhibitory effects of callus, in vivo and in vitro leaf methanol extracts. The inhibition rate was mainly dependent on concentration of extracts. Total phenols, tannins and flavonoids contents of the test samples were quantified. The overall results from the study highlights the use of least concentrations of methanolic callus extracts of plant has potential use in treatment of cancer related diseases in future.
Keywords: ABT’S, Aerva lanata, Callus, DNA fragmentation, MCF – 7, MTT.
Graphical Abstract
The Natural Products Journal
Title:Antiproliferative and Antioxidant Potential of Leaf and Leaf Derived Callus Extracts of Aerva lanata (L.) Juss. Ex Schult. Against Human Breast Cancer (MCF-7) Cell Lines
Volume: 4 Issue: 4
Author(s): Deepak Kamalanathan and Devarajan Natarajan
Affiliation:
Keywords: ABT’S, Aerva lanata, Callus, DNA fragmentation, MCF – 7, MTT.
Abstract: Aerva lanata is a prostrate under-shrub used as traditional and modern system of medicine. The Prime of current study was to compare the antiproliferative and antioxidant potential of in vitro and in vivo leaf and callus extracts of Aerva lanata against human breast cancer (MCF 7) cell lines. Anticancer proficiency of methanol extracts of in vitro and in vivo leaf and matured callus extracts were evaluated by 3 - (4, 5 – dimethylthiazole – 2 – yl) - 2, 5 -diphenyl tetrazolium bromide (MTT) and DNA fragmentation assays. Earlier the total antioxidant potential of the different solvent extracts was tested by 2, 2' – azino – bis (3-ethylbenzthiazoline – 6 – sulphonic acid (ABTS) assay. A dose dependent inhibition of cancer cell proliferation was identified in MTT assay. Further, DNA fragmentation assay was performed and the results show the best IC50 values produced best possible digestion of DNA (by apoptosis). The results of radical scavenging activity of ABTS cation assay was recorded promising inhibitory effects of callus, in vivo and in vitro leaf methanol extracts. The inhibition rate was mainly dependent on concentration of extracts. Total phenols, tannins and flavonoids contents of the test samples were quantified. The overall results from the study highlights the use of least concentrations of methanolic callus extracts of plant has potential use in treatment of cancer related diseases in future.
Export Options
About this article
Cite this article as:
Kamalanathan Deepak and Natarajan Devarajan, Antiproliferative and Antioxidant Potential of Leaf and Leaf Derived Callus Extracts of Aerva lanata (L.) Juss. Ex Schult. Against Human Breast Cancer (MCF-7) Cell Lines, The Natural Products Journal 2014; 4 (4) . https://dx.doi.org/10.2174/2210315504666141119223215
DOI https://dx.doi.org/10.2174/2210315504666141119223215 |
Print ISSN 2210-3155 |
Publisher Name Bentham Science Publisher |
Online ISSN 2210-3163 |

- Author Guidelines
- Bentham Author Support Services (BASS)
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
New Developments and Prospective Applications for β (1,3) Glucans
Recent Patents on Biotechnology Autoimmune Diseases in Gastroenterology
Current Pharmaceutical Design Radio-localization of Non-Palpable Breast Lesions Under Ultrasonographic Guidance: Current Status and Future Perspectives
Current Radiopharmaceuticals Quantitative Imaging Biomarkers and their Emerging Role
Current Medical Imaging Oligonucleotide Aptamers for Glioma Targeting: An Update
Central Nervous System Agents in Medicinal Chemistry Learning from Nature: Bioinspired Strategies Towards Antimicrobial Nanostructured Systems
Current Topics in Medicinal Chemistry TRPV1 Function in Health and Disease
Current Pharmaceutical Biotechnology The Antiprogestogen Mifepristone: A Review
Current Medicinal Chemistry - Immunology, Endocrine & Metabolic Agents Micro-flow Immunosensor Based on Thin-film Interdigitated Gold Array Microelectrodes for Cancer Biomarker Detection
Current Drug Delivery Biological Interfacial Engineering for Metastatic Cancer Diagnosis and Intervention
Current Medicinal Chemistry Recent Developments in Patents Targeting Toll-Like Receptor Genes
Recent Patents on DNA & Gene Sequences Cancer Diagnosis and Treatment Guidance: Role of MRI and MRI Probes in the Era of Molecular Imaging
Current Pharmaceutical Biotechnology Current Trends in Cancer Biomarker Discovery Using Urinary Metabolomics: Achievements and New Challenges
Current Medicinal Chemistry Randomized Controlled Clinical Trials in Mild-Moderate Ulcerative Colitis
Reviews on Recent Clinical Trials Benzocoumarins: Isolation, Synthesis, and Biological Activities
Mini-Reviews in Medicinal Chemistry Changes in the Expression of the Alzheimers Disease-Associated Presenilin Gene in Drosophila Heart Leads to Cardiac Dysfunction
Current Alzheimer Research Magnetic Resonance Spectroscopy Biomarkers in Term Perinatal Asphyxial Encephalopathy: From Neuropathological Correlates to Future Clinical Applications
Current Pediatric Reviews Targeted Gene Therapy for Ovarian Cancer
Current Gene Therapy Regulation of Autophagy in Oxygen-Dependent Cellular Stress
Current Pharmaceutical Design Importance of Influx and Efflux Systems and Xenobiotic Metabolizing Enzymes in Intratumoral Disposition of Anticancer Agents
Current Cancer Drug Targets