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Anti-Cancer Agents in Medicinal Chemistry

Editor-in-Chief

ISSN (Print): 1871-5206
ISSN (Online): 1875-5992

Selection and Characterization of Human Anti-MAGE-A1 scFv and Immunotoxin

Author(s): Hong Lin, Yuan Mao, Da-Wei Zhang, Hu Li, Jin-Rong Qiu, Jin Zhu and Ren-Jie Chen

Volume 13, Issue 8, 2013

Page: [1259 - 1266] Pages: 8

DOI: 10.2174/18715206113139990134

Price: $65

Abstract

Melanoma-associated antigen (MAGE) is expressed on the surface of multiple tumor cell types and is a promising target of biotherapeutic drug delivery via the anti-MAGE-A1 antibody. In this study, a human single-chain variable fragment (scFv) antibody phage library was generated and applied to recombinant MAGE-A1-coated immunotubes by phage display technology. The soluble anti- MAGE-A1 scFv was expressed and purified by immobilized metal-chelated affinity chromatography (IMAC). The anti-MAGE-A1 scFv could bind native MAGE-A1 confirmed by enzyme-linked immunosorbent assay (ELISA), dot blot, and immunoprecipitation (IP) analysis. The immunotoxin was expressed and purified by IMAC successfully. The results indicated that the human anti-MAGE-A1 immunotoxin could provide a valuable drug for clinic cancer therapy.

Keywords: Phage display antibody library, anti-MAGE-A1 scFv fragment, immunotoxin.


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