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Protein & Peptide Letters

Editor-in-Chief

ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Production of Minichaperone (sht GroEL191-345) and Its Function in the Refolding of Recombinant Human Interferon Gamma

Author(s): Yi-Xin Guan, Zheng-Zheng Fei, Man Luo, Shan-Jing Yao and Man-Gi Cho

Volume 12, Issue 1, 2005

Page: [85 - 88] Pages: 4

DOI: 10.2174/0929866053405995

Price: $65

Abstract

The recombinant minichaperone sht GroEL191-345 was cultivated in a 3.7 L stirred bioreactor with the high yield of 216.2 mg / L broth. In the refolding of recombinant human interferon gamma (rhuIFN-γ) inclusion bodies, more than 2-3 fold enhancement in protein mass recovery and total activity were observed in the presence of free or immobilized minichaperone to the refolding buffer.

Keywords: minichaperone, sht groel191-345, cultivation, purification, refolding, recombinant human interferon gamma


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