Chitinase-Like Proteins with Antifungal Activity from Emperor Banana Fruits

Title: Chitinase-Like Proteins with Antifungal Activity from Emperor Banana Fruits

Volume: 14 Issue: 8

Author(s): Vincent S.M. Ho and Tzi Bun Ng

Affiliation:

Keywords: Emperor banana, chitinases, isolation

Abstract: Two 30-kDa proteins with N-terminal sequence homology to chitinases have been isolated from fruits of the emperor banana by using a protocol that involved (NH4)2SO4 precipitation, affinity chromatography on Affi-gel blue gel, ion exchange chromatography by fast protein liquid chromatography (FPLC) on Mono S and gel filtration by FPLC on Superdex 75. The proteins were adsorbed on Affi-gel blue gel and Mono S. They both inhibited mycelial growth in Fusarium oxysporum but not in Mycosphaerella arachidicola. The chitinase-like protein more strongly bound on Mono S was obtained with a slightly lower yield and exhibited a higher antifungal potency toward F. oxysporum when compared with the less strongly bound chitinase-like protein.

Cite this article as:

Vincent S.M. Ho and Tzi Bun Ng , Chitinase-Like Proteins with Antifungal Activity from Emperor Banana Fruits, Protein & Peptide Letters 2007; 14 (8) . https://dx.doi.org/10.2174/092986607781483750

DOI https://dx.doi.org/10.2174/092986607781483750	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305