Expression, Purification and Characterisation of Haemophilus influenzae 3-Isopropylmalate Dehydrogenase (LeuB)

Title: Expression, Purification and Characterisation of Haemophilus influenzae 3-Isopropylmalate Dehydrogenase (LeuB)

Volume: 14 Issue: 8

Author(s): Sara Martignon, Franca Rossi and Menico Rizzi

Affiliation:

Keywords: Haemophilus influenzae, 3-isopropylmalate dehydrogenase, recombinant enzyme, leucine biosynthesis, antibacterial target, product inhibition

Abstract: 3-isopropylmalate dehydrogenase (3IPMDH) is the third enzyme in leucine biosynthesis and a promising target for the development of broad-spectrum antibacterial agents. We report here the expression, purification and biochemical characterisation of Haemophilus influenzae 3-isopropylmalate dehydrogenase. The observed enzyme inhibition by the reaction product NADH could represent a regulatory mechanism for 3IPMDH.

Cite this article as:

Sara Martignon , Franca Rossi and Menico Rizzi , Expression, Purification and Characterisation of Haemophilus influenzae 3-Isopropylmalate Dehydrogenase (LeuB), Protein & Peptide Letters 2007; 14 (8) . https://dx.doi.org/10.2174/092986607781483606