Login Register Cart 0
Generic placeholder image

Current Enzyme Inhibition

Editor-in-Chief

ISSN (Print): 1573-4080
ISSN (Online): 1875-6662

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

Antioxidant and Tyrosinase Inhibitory Activities of Athamanta sicula L. Aqueous Extract

Author(s): Karima Loucif*, Fatima Benchikh, Hassiba Benabdallah, Chawki Bensouici and Smain Amira

Volume 19, Issue 2, 2023

Published on: 27 June, 2023

Page: [81 - 86] Pages: 6

DOI: 10.2174/1573408018666220322163312

Price: $65

conference banner
Abstract

Introduction: Overexpression of tyrosinase in humans causes an increase in melanin production in the skin, which can result in hyperpigmentation consequences such as freckles, melasma, age spots, and melanoma. Free radicals also play a significant role in the increase of the biosynthesis of melanin. Tyrosinase inhibitors capable of inhibiting the biosynthesis of melanin are currently used in various hyperpigmentation and cosmetic agents to control the formation of freckles. Several synthetic tyrosinase inhibitors have been associated with several serious side effects. Also, synthetic antioxidants have many toxicological side effects, including carcinogenicity. There is an increasing interest in the search for natural tyrosinase inhibitors and antioxidant agents.

Aims: The objective of this study is to evaluate total polyphenol and flavonoid contents as well as examine the antioxidative and tyrosinase inhibitory effects of A. sicula L. aqueous extract.

Methods: Antioxidant activities were evaluated using superoxide radical scavenging and reducing power methods. Moreover, a tyrosinase inhibitory assay was used to determine anti-hyperpigmentation.

Results: The results showed that this extract was rich in total polyphenols (58.01 ± 1.18 micrograms of gallic acid equivalents per milligrams of extract) and flavonoids (17.91 ± 1.81 micrograms quercetin equivalents per milligram of extract). A. sicula L. aqueous extract was capable of scavenging free radicals (IC50 = 11.87 ± 0.13 μg/mL) and acting as a strong reducing agent (A 0.5= 6.37 ± 0.42 μg/mL). A. sicula L. had a potent tyrosinase inhibitory potential (IC50= 12.63 ± 1.15 μg/mL), which was higher compared to kojic acid used as a standard (IC50= 25,23 ± 0,78 μg/mL, p <0.001).

Conclusion: These results support that A. sicula L. could be a new source of antioxidant and cosmetic use. Further studies focusing on the isolation and characterization of active principles of antioxidant and tyrosinase inhibitory activities are needed.

Keywords: Athamanta sicula L., phenolic compounds, antioxidant properties, tyrosinase inhibitory activity, inflammation, hyperpigmentation.

« Previous Next »
Graphical Abstract

[1]
Cui HX, Duan FF, Jia SS, Cheng FR, Yuan K. Antioxidant and tyrosinase inhibitory activities of seed oils from Torreya grandis Fort. ex Lindl. BioMed Res Int 2018; 2018: 5314320.
[http://dx.doi.org/10.1155/2018/5314320] [PMID: 30320135]
[2]
Yamaguchi Y, Brenner M, Hearing VJ. The regulation of skin pigmentation. J Biol Chem 2007; 282(38): 27557-61.
[http://dx.doi.org/10.1074/jbc.R700026200] [PMID: 17635904]
[3]
Loucif K, Benabdallah H, Benchikh F, et al. Metal chelating and cupric ion reducing antioxidant capacities of Ammoides atlantica aqueous extract. J Drug Deliv Ther 2020; 10(4): 108-11.
[http://dx.doi.org/10.22270/jddt.v10i4-s.4245]
[4]
Alam N, Yoon KN, Lee JS, Cho HJ, Lee TS. Consequence of the antioxidant activities and tyrosinase inhibitory effects of various extracts from the fruiting bodies of Pleurotus ferulae. Saudi J Biol Sci 2012; 19(1): 111-8.
[http://dx.doi.org/10.1016/j.sjbs.2011.11.004] [PMID: 23961169]
[5]
Peng LH, Liu S, Xu SY, et al. Inhibitory effects of salidroside and paeonol on tyrosinase activity and melanin synthesis in mouse B16F10 melanoma cells and ultraviolet B-induced pigmentation in guinea pig skin. Phytomedicine 2013; 20(12): 1082-7.
[http://dx.doi.org/10.1016/j.phymed.2013.04.015] [PMID: 23746955]
[6]
Wang GH, Chen CY, Lin CP, et al. Tyrosinase inhibitory and antioxidant activities of three Bifidobacterium bifidum-fermented herb extracts. Ind Crops Prod 2016; 89: 376-82.
[http://dx.doi.org/10.1016/j.indcrop.2016.05.037]
[7]
Saqib F, Janbaz KH, Sherwani MK. In vitro inhibitory potential of methanolic extract of Celosia argentea var. cristata on tyrosinase, acetylcholinesterase and butyrylcholinesterase enzymes. Bangladesh J Pharmacol 2015; 10(2): 449-54.
[http://dx.doi.org/10.3329/bjp.v10i2.22880]
[8]
Solimine J, Garo E, Wedler J, et al. Tyrosinase inhibitory constituents from a polyphenol enriched fraction of rose oil distillation waste water. Fitoterapia 2016; 108: 13-9.
[http://dx.doi.org/10.1016/j.fitote.2015.11.012] [PMID: 26592852]
[9]
Lin YS, Chen SH, Huang WJ, et al. Effects of nicotinic acid derivatives on tyrosinase inhibitory and antioxidant activities. Food Chem 2012; 132(4): 2074-80.
[http://dx.doi.org/10.1016/j.foodchem.2011.12.052]
[10]
Neeley E, Fritch G, Fuller A, Wolfe J, Wright J, Flurkey W. Variations in IC(50) values with purity of mushroom tyrosinase. Int J Mol Sci 2009; 10(9): 3811-23.
[http://dx.doi.org/10.3390/ijms10093811] [PMID: 19865520]
[11]
Ando H, Matsui MS, Ichihashi M. Quasi-drugs developed in Japan for the prevention or treatment of hyperpigmentary disorders. Int J Mol Sci 2010; 11(6): 2566-75.
[http://dx.doi.org/10.3390/ijms11062566] [PMID: 20640168]
[12]
Ortonne JP. Normal and abnormal skin color. Ann Dermatol Venereol 2012; 139 (Suppl. 4): S125-9.
[http://dx.doi.org/10.1016/S0151-9638(12)70123-0] [PMID: 23522626]
[13]
Loucif K, Benabdallah H, Benchikh F, Smain A. Evalution polyphenol contents and antioxidant capacities by DPPH and phenanthroline antioxidant assays from hydromethanolic extract of Athamanta sicula L. 2019. d
[14]
Loucif K, Benabdallah H, Benchikh F, Mehlous S, Souici CB, Amira S. Total phenolic contents, DPPH radical scavenging and β-Carotene bleaching activities of aqueous extract from Ammoides atlantica. J Drug Deliv Ther 2020; 10(3): 196-8.
[http://dx.doi.org/10.22270/jddt.v10i3-s.4151]
[15]
Labed I, Chibani S, Semra Z, et al. Antibacterial activity and chemical composition of essential oil of Athamanta sicula L.(Apiaceae) from Algeria. J Chem 2012; 9(2): 796-800.
[http://dx.doi.org/10.1155/2012/963719]
[16]
Stefano VD, Pitonzo R, Schillaci D. Antimicrobial and antiproliferative activity of Athamanta sicula L. (Apiaceae). Pharmacogn Mag 2011; 7(25): 31-4.
[http://dx.doi.org/10.4103/0973-1296.75893] [PMID: 21472076]
[17]
Ferreira A, Proença C, Serralheiro MLM. Araújo MEM. The in vitro screening for acetylcholinesterase inhibition and antioxidant activity of medicinal plants from Portugal. J Ethnopharmacol 2006; 108(1): 31-7.
[http://dx.doi.org/10.1016/j.jep.2006.04.010] [PMID: 16737790]
[18]
Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 1965; 16(3): 144-58.
[19]
Topçu G, Ay M, Bilici A. Sarıkürkcü C, Öztürk M, Ulubelen A. A new flavone from antioxidant extracts of Pistacia terebinthus. Food Chem 2007; 103(3): 816-22.
[http://dx.doi.org/10.1016/j.foodchem.2006.09.028]
[20]
Elizabeth K, Rao MNA. Oxygen radical scavenging activity of curcumin. Int J Pharm 1990; 58: 237-40.
[http://dx.doi.org/10.1016/0378-5173(90)90201-E]
[21]
Bouratoua A, Khalfallah A, Bensouici C, et al. Chemical composition and antioxidant activity of aerial parts of Ferula longipes Coss. ex Bonnier and Maury. Nat Prod Res 2018; 32(16): 1873-80.
[http://dx.doi.org/10.1080/14786419.2017.1353513] [PMID: 28714345]
[22]
Deveci E, Tel-Çayan G, Duru ME. Phenolic profile, antioxidant, anticholinesterase and antityrosinase activities of the various extracts of Ferulaelaeochytris and Sideritis stricta. Int J Food Prop 2018; 21(1): 771-83.
[http://dx.doi.org/10.1080/10942912.2018.1431660]
[23]
Chung YC, Chang CT, Chao WW, Lin CF, Chou ST. Antioxidative activity and safety of the 50 ethanolic extract from red bean fermented by Bacillus subtilis IMR-NK1. J Agric Food Chem 2002; 50(8): 2454-8.
[http://dx.doi.org/10.1021/jf011369q] [PMID: 11929313]
[24]
Bendjabeur S, Benchabane O, Bensouici C, Hazzit M, Baaliouamer A, Bitam A. Antioxidant and anticholinesterase activity of essential oils and ethanol extracts of Thymus algeriensis and Teucrium polium from Algeria. J Food Meas Charact 2018; 12(4): 2278-88.
[http://dx.doi.org/10.1007/s11694-018-9845-x]
[25]
Chun OK, Kim DO, Lee CY. Superoxide radical scavenging activity of the major polyphenols in fresh plums. J Agric Food Chem 2003; 51(27): 8067-72.
[http://dx.doi.org/10.1021/jf034740d] [PMID: 14690398]
[26]
Kim YJ, Uyama H. Tyrosinase inhibitors from natural and synthetic sources: structure, inhibition mechanism and perspective for the future. Cell Mol Life Sci 2005; 62(15): 1707-23.
[http://dx.doi.org/10.1007/s00018-005-5054-y] [PMID: 15968468]
[27]
Adhikari A, Devkota HP, Takano A, et al. Screening of Nepalese crude drugs traditionally used to treat hyperpigmentation: In vitro tyrosinase inhibition. Int J Cosmet Sci 2008; 30(5): 353-60.
[http://dx.doi.org/10.1111/j.1468-2494.2008.00463.x] [PMID: 18822041]
[28]
Kamagaju L, Morandini R, Bizuru E, et al. Tyrosinase modulation by five Rwandese herbal medicines traditionally used for skin treatment. J Ethnopharmacol 2013; 146(3): 824-34.
[http://dx.doi.org/10.1016/j.jep.2013.02.010] [PMID: 23439030]
[29]
Panzella L, Napolitano A. Natural and bioinspired phenolic compounds as tyrosinase inhibitors for the treatment of skin hyperpigmentation: Recent advances. Cosmetics 2019; 6(4): 57.
[http://dx.doi.org/10.3390/cosmetics6040057]

Rights & Permissions Print Cite
Article Metrics
1
© 2024 Bentham Science Publishers | Privacy Policy