Login Register Cart 0
Generic placeholder image

CNS & Neurological Disorders - Drug Targets

Editor-in-Chief

ISSN (Print): 1871-5273
ISSN (Online): 1996-3181

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

Zederone Improves the Fecal Microbial Profile in Dementia Induced Rat Model: A First Report

Author(s): Sudarshana Borah, Priyanka Sarkar and Hemanta Kumar Sharma*

Volume 21, Issue 4, 2022

Published on: 27 August, 2021

Page: [335 - 342] Pages: 8

DOI: 10.2174/1871527320666210827114227

Price: $65

Abstract

Background: Dementia correlates with Alzheimer’s disease, Parkinson’s disease, frontotemporal and cerebrovascular diseases. There are supporting shreds of evidence on the pharmacological activity of curcuma caesia (Zingiberaceae family) for its antioxidant, antidepressant, analgesic, anticonvulsant, and anti-acetylcholinesterase effect.

Objective: This study aims to analyze the fecal microbial profile in Zederone treated demented rat model.

Methods: In our study, isolation and characterization of Zederone were carried out from curcuma caesia rhizomes, followed by estimation of its memory-enhancing effect on Aluminium-induced demented rat, which was evaluated by behavioural study on radial 8 arm maze. Moreover the detection of amyloid plaque formation was carried out using fluorescent microscopy of the congo red-stained rat brain tissues of the cerebral neocortex region. This study included eighteen female Wistar Albino rats that were divided into three groups that consisted of six rats in each group. The study of fecal microbial profile by metagenomic DNA extraction followed by next-generation sequencing was carried out to establish the correlation between gut microbes and amyloid plaques in dementia.

Results: Zederone could be characterized as pale yellow colored, needle-shaped crystals with 96.57% purity. This compound at 10 mg/kg body weight showed cognition improving capacity (p ≤ 0.0001) with a reduction of accumulated amyloid plaques that were detected in the demented group in fluorescence microscope and fecal microbiome study divulged an increased shift towards Lactobacillus genera in the treated group from Bacteroides in the demented group.

Conclusion: This sesquiterpenoid compound would assist in the modulation of gut bacterial dysbiosis and act as a better therapeutic drug for dementia and other neurological disorders.

Keywords: Alzheimer’s disease, gut bacteriome, DNA, metagenomic, Bacteroides, Lactobacillus.

« Previous Next »
Graphical Abstract

[1]
Shaji KS, Jithu VP, Jyothi KS. Indian research on aging and dementia. Indian J Psychiatry 2010; 52(Suppl. 1): S148-52.
[http://dx.doi.org/10.4103/0019-5545.69227] [PMID: 21836672]
[2]
Cunningham EL, McGuinness B, Herron B, Passmore AP. Dementia. Ulster Med J 2015; 84(2): 79-87.
[PMID: 26170481]
[3]
Tremlett H, Bauer KC, Appel-Cresswell S, Finlay BB, Waubant E. The gut microbiome in human neurological disease: A review. Ann Neurol 2017; 81(3): 369-82.
[http://dx.doi.org/10.1002/ana.24901] [PMID: 28220542]
[4]
Sainaghi PP, Bellan M, Lombino F, et al. Growth arrest specific 6 concentration is increased in the cerebrospinal fluid of patients with Alzheimer’s disease. J Alzheimers Dis 2017; 55(1): 59-65.
[http://dx.doi.org/10.3233/JAD-160599] [PMID: 27636849]
[5]
Comi C, Tondo G. Insights into the protective role of immunity in neurodegenerative disease. Neural Regen Res 2017; 12(1): 64-5.
[http://dx.doi.org/10.4103/1673-5374.198980] [PMID: 28250745]
[6]
Cappellano G, Carecchio M, Fleetwood T, et al. Immunity and inflammation in neurodegenerative diseases. Am J Neurodegener Dis 2013; 2(2): 89-107.
[PMID: 23844334]
[7]
Arulmozhi DK, Sridhar N, Veeranjaneyulu A, Arora SK. Preliminary mechanistic studies on the smooth muscle relaxant effect of hydroalcoholic extract of Curcuma caesia. J Herb Pharmacother 2006; 6(3-4): 117-24.
[http://dx.doi.org/10.1080/J157v06n03_06] [PMID: 17317653]
[8]
Karmakar I, Dolai N, Saha P, Sarkar N, Bala A, Haldar PK. Scavenging activity of Curcuma caesia rhizome against reactive oxygen and nitrogen species. Orient Pharm Exp Med 2011; 11: 221-8.
[http://dx.doi.org/10.1007/s13596-011-0030-6]
[9]
Borah S, Sarkar P, Sharma HK. Chemical profiling, free radical scavenging and anti - acetylcholinesterase activities of essential oil from Curcuma caesia of Arunachal Pradesh, India. Pharmacogn Rev 2020; 12: 76-84.
[http://dx.doi.org/10.4103/pr.pr_84_19]
[10]
Li Y, Teng Z, Parkin KL, et al. Identification of bioactive metabolites dihydrocanadensolide, Kojic acid, and vanillic acid in soy sauce using GC-MS, NMR spectroscopy, and single-crystal X-ray diffraction. J Agric Food Chem 2014; 62(33): 8392-401.
[http://dx.doi.org/10.1021/jf502159m] [PMID: 25090452]
[11]
Borah S, Sharma HK. Isolation, characterization of compound from Curcuma caesia roxb., with special reference to anti-alzheimer's study: Recent discoveries in medicinal and aromatic plants in north east India. In: India: Eastern Book House Publisher 2019; pp. 33-40.
[12]
Mradu G, Saumyakanti S, Sohini M, Arup M. HPLC profiles of standard phenolic compounds present in medicinal plants. Int J Pharmacogn Phytochem Res 2012; 4: 162-7.
[13]
Organization for economic co-operation and development (OECD) guideline for testing of chemicals 423: Acute oral toxicity - acute toxic class method. 2001.
[14]
Singhal KG, Das Gupta G. Neuroprotective appraisal of methanolic extract of flowers of Nerium oleander in a non classical rat model of alzheimer disease. Nat Prod J 2012; 2: 235-45.
[http://dx.doi.org/10.2174/2210315511202030235]
[15]
Borah S, Sarkar P, Sharma HK. Therapeutic potential of Curcuma caesia rhizome against neurodegenerative disease. Curr Trend Pharm Res 2020; 6: 116-9.
[16]
Caporaso JG, Lauber CL, Walters WA, et al. Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample. Proc Natl Acad Sci USA 2011; 108(Suppl. 1): 4516-22.
[http://dx.doi.org/10.1073/pnas.1000080107] [PMID: 20534432]
[17]
Quast C, Pruesse E, Yilmaz P, et al. The SILVA ribosomal RNA gene database project: Improved data processing and web-based tools. Nucleic Acids Res 2013; 41: D590-6.
[PMID: 23193283]
[18]
Allec SI, Sun Y, Sun J, Chang CA, Wong BM. Heterogeneous CPU+GPU- Enabled simulations for DFTB molecular dynamics of large chemical and biological systems. J Chem Theory Comput 2019; 15(5): 2807-15.
[http://dx.doi.org/10.1021/acs.jctc.8b01239] [PMID: 30916958]
[19]
Lemkul JA, Bevan DR. The role of molecular simulations in the development of inhibitors of amyloid β-peptide aggregation for the treatment of Alzheimer’s disease. ACS Chem Neurosci 2012; 3(11): 845-56.
[http://dx.doi.org/10.1021/cn300091a] [PMID: 23173066]
[20]
Syed AK, Boles BR. Fold modulating function: Bacterial toxins to functional amyloids. Front Microbiol 2014; 5: 401.
[http://dx.doi.org/10.3389/fmicb.2014.00401] [PMID: 25136340]
[21]
Vogt NM, Kerby RL, Dill-McFarland KA, et al. Gut microbiome alterations in Alzheimer’s disease. Sci Rep 2017; 7(1): 13537.
[http://dx.doi.org/10.1038/s41598-017-13601-y] [PMID: 29051531]
[22]
Kobayashi Y, Sugahara H, Shimada K, et al. Therapeutic potential of Bifidobacterium breve strain A1 for preventing cognitive impairment in Alzheimer’s disease. Sci Rep 2017; 7(1): 13510.
[http://dx.doi.org/10.1038/s41598-017-13368-2] [PMID: 29044140]
[23]
Magistrelli L, Amoruso A, Mogna L, et al. Probiotics may have beneficial effects in Parkinson’s disease: In vitro evidence. Front Immunol 2019; 10: 969.
[http://dx.doi.org/10.3389/fimmu.2019.00969] [PMID: 31134068]
[24]
Liu X, Cao S, Zhang X. Modulation of gut microbiota-brain axis by Probiotics, prebiotics and diets. J Agric Food Chem 2015; 63(36): 7885-95.
[http://dx.doi.org/10.1021/acs.jafc.5b02404] [PMID: 26306709]
[25]
Wood JD. Enteric neuroimmunophysiology and pathophysiology. Gastroenterology 2004; 127(2): 635-57.
[http://dx.doi.org/10.1053/j.gastro.2004.02.017] [PMID: 15300595]
[26]
Woo JY, Gu W, Kim KA, Jang SE, Han MJ, Kim DH. Lactobacillus pentosus var. plantarum C29 ameliorates memory impairment and inflammaging in a D-galactose-induced accelerated aging mouse model. Anaerobe 2014; 27: 22-6.
[http://dx.doi.org/10.1016/j.anaerobe.2014.03.003] [PMID: 24657159]
[27]
Athari Nik Azm S, Djazayeri A, Safa M, et al. Lactobacilli and bifidobacteria ameliorate memory and learning deficits and oxidative stress in β-amyloid (1-42) injected rats. Appl Physiol Nutr Metab 2018; 43(7): 718-26.
[http://dx.doi.org/10.1139/apnm-2017-0648] [PMID: 29462572]
[28]
Deshpande NG, Saxena J, Pesaresi TG, et al. High fat diet alters gut microbiota but not spatial working memory in early middle-aged Sprague Dawley rats. PLoS One 2019; 14(5): e0217553.
[http://dx.doi.org/10.1371/journal.pone.0217553] [PMID: 31141574]
[29]
Eckburg PB, Bik EM, Bernstein CN, et al. Diversity of the human intestinal microbial flora. Science 2005; 308(5728): 1635-8.
[http://dx.doi.org/10.1126/science.1110591] [PMID: 15831718]
[30]
Gill SR, Pop M, Deboy RT, et al. Metagenomic analysis of the human distal gut microbiome. Science 2006; 312(5778): 1355-9.
[http://dx.doi.org/10.1126/science.1124234] [PMID: 16741115]

Rights & Permissions Print Cite
Article Metrics
28
3
© 2024 Bentham Science Publishers | Privacy Policy