Login Register Cart 0
Generic placeholder image

Combinatorial Chemistry & High Throughput Screening

Editor-in-Chief

ISSN (Print): 1386-2073
ISSN (Online): 1875-5402

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

Validation of an UPLC-DAD Method for the Quantification of Phenolic Acids, Verbascoside and 6-epi-aucubin in Crescentia cujete Fruit

Author(s): Andrés Rivera-Mondragón*, Catherina Caballero-George, Luc Pieters and Kenn Foubert

Volume 25, Issue 7, 2022

Published on: 07 December, 2020

Page: [1148 - 1157] Pages: 10

DOI: 10.2174/1386207323666201207114302

Price: $65

Abstract

Background: The fruit pulp decoction of Crescentia cujete, commonly known as calabash, is traditionally used for the treatment of several respiratory diseases and is available as syrup formulations. Unfortunately, there is no detailed investigation on the analytical methods for warranting the quality of these products.

Aims and objectives: To develop and validate an appropriate analytical method for the simultaneous quantification of trans-cinnamic acid, 4-hydroxybenzoic acid, verbascoside and 6- epi-aucubin in the decoction and commercial cough syrups of Crescentia cujete fruit.

Materials and Methods: A reversed-phase ultra-high-performance liquid chromatographic method coupled to a diode array detector (UPLC-DAD) was validated following the ICH guidelines. The chromatographic analysis was performed using a C18 column, the mobile phase system consisted of water and acetonitrile containing 0.1% formic acid, and UV chromatograms were recorded from 200 to 400 nm.

Results: A new UPLC-DAD method was validated for the simultaneous quantification of transcinnamic acid, 4-hydroxybenzoic acid, verbascoside and 6-epi-aucubin in calabash-derived products. After successful validation, this method was applied for the quantification of the selected chemical markers in an in-house decoction and three commercial cough syrups. Among the selected chemical markers, 6-epi-aucubin was the main compound in the calabash decoction, while trans-cinnamic acid and 4-hydroxybenzoic acid were the major compounds in the commercial products. Verbascoside and 6-epi-aucubin were below the limit of quantification in all syrup samples.

Conclusion: The proposed method was successfully applied for the analysis of three commercial syrup formulations and can be useful for standardization and quality control of raw and pharmaceutical calabash preparations.

Keywords: Crescentia cujete, bignoniaceae, UPLC-DAD, phenolic acids, verbascoside, iridoid glycosides, decoctions, cough syrups.

« Previous Next »
Graphical Abstract

[1]
Gentry, A.H. Bignoniaceae: Part I (Crescentiae and Tourrrettiaeae); New York Botanical Garden Press: New York, 1980.
[2]
Ejelonu, B.C.; Lasisi, A.A.; Olaremu, A.G.; Ejelonu, O.C. The chemical constituents of calabash (Crescentia cujete). Afr. J. Biotechnol., 2011, 10, 19631-19636.
[http://dx.doi.org/10.5897/AJB11.1518]
[3]
Rivera-Mondragón, A.; Tuenter, E.; Ortiz, O.; Sakavitsi, M.E.; Nikou, T.; Halabalaki, M.; Caballero-george, C.; Apers, S.; Pieters, L.; Foubert, K. UPLC-MS/MS-based molecular networking and NMR structural determination for the untargeted phytochemical characterization of the fruit of Crescentia cujete (Bignonaceae). Phytochemistry, 2020, 177112438
[http://dx.doi.org/10.1016/j.phytochem.2020.112438]
[4]
Kaneko, T.; Ohtani, K.; Kasai, R.; Yamasaki, K.; Neguyen, M.D. Iridoids and iridoid glucosides from fruits of Crescentia cujete. Phytochemistry, 1997, 46, 907-910.
[http://dx.doi.org/10.1016/S0031-9422(97)00375-0]
[5]
Chen, Y.; Tadey, T.; Hu, M.; Carr, G.; Guo, J. Quantitative HPLC analysis of active pharmaceutical ingredients in syrup vehicle using centrifugal filter devices and determination of xanthan gum in syrup vehicle using rheometry. J. Chromatogr. Sci., 2010, 48(2), 104-108.
[http://dx.doi.org/10.1093/chromsci/48.2.104] [PMID: 20109286]
[6]
International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use, Validation of Analytical Procedures: (ICH) Q2 (R1): Text and methodology 2005.
[7]
Analytical Procedures and Methods Validation for Drugs and Biologics; Rockville, USA, 2015.
[8]
Bhandari, P; Kumar, N; Gupta, AP; Singh, B; Kaul, VK A rapid RP-HPTLC densitometry method for simultaneous determination of major flavonoids in important medicinal plants., 2007, 2092-2096.
[http://dx.doi.org/10.1002/jssc.200700066]
[9]
Funk, W.; Dammann, V.; Donnevert, G. Quality Assurance in Analytical Chemistry: Applications in Environmental, Food and Materials Analysis, Biotechnology, and Medical Engineering, 2nd ed; Wiley-VCH: Weinheim, Germany, 2007.
[10]
Van Loco, J.; Elskens, M.; Croux, C.; Beernaert, H. Linearity of calibration curves: Use and misuse of the correlation coefficient. Accredit. Qual. Assur., 2002, 7, 281-285.
[http://dx.doi.org/10.1007/s00769-002-0487-6]
[11]
Decleer, M.; Rajkovic, A.; Sas, B.; Madder, A.; De Saeger, S. Development and validation of ultra-high-performance liquid chromatography-tandem mass spectrometry methods for the simultaneous determination of beauvericin, enniatins (A, A1, B, B1) and cereulide in maize, wheat, pasta and rice. J. Chromatogr. A, 2016, 1472, 35-43.
[http://dx.doi.org/10.1016/j.chroma.2016.10.003] [PMID: 27776774]
[12]
2002.https://publications.europa.eu/en/publication-detail/-/public-ation/ed928116-a955-4a84-b10a-cf7a82bad858/language-en
[13]
Quality control methods for herbal materials; WHO Press: Geneva, Switzerland, 2011, pp. 1-137.
[14]
González, A.G.; Herrador, M.Á. A practical guide to analytical method validation, including measurement uncertainty and accuracy profiles. Trends Analyt. Chem., 2007, 26, 227-238.
[http://dx.doi.org/10.1016/j.trac.2007.01.009]
[15]
Chow, S.C.; Zheng, J. The use of 95% CI or 90% CI for drug product development — a controversial issue? J. Biopharm. Stat., 2019, 29, 834-844.
[http://dx.doi.org/10.1080/10543406.2019.1657141]
[16]
Belouafa, S.; Habti, F.; Benhar, S.; Belafkih, B.; Tayane, S.; Hamdouch, S.; Bennamara, A.; Abourriche, A. Statistical tools and approaches to validate analytical methods: Methodology and practical examples; Int J Metrol Qual Eng, 2017, p. 8.
[17]
Smith, G. European Medicines Agency. Commitee for Medicinal Products for Human Use (CHMP). Guideline ob bioanalytical method validation. EMEA/CHMP/EWP/192217/2009 Rev. 1 Corr, 2012.
[18]
Ermer, J.; Miller, J.H.M. Method Validation in Pharmaceutical Analysis: A Guide to Best Practice, 1st ed; Wiley-VCH: Weinheim, Germany, 2005.
[http://dx.doi.org/10.1002/3527604685]
[19]
Kaneko, T.; Ohtani, K.; Kasai, R.; Yamasaki, K.; Neguyen, M.D. n-Alkyl glycosides and p-hydroxybenzoyloxy glucose from fruits of Crescentia cujete. Phytochemistry, 1998, 47, 259-263.
[http://dx.doi.org/10.1016/S0031-9422(97)00409-3]
[20]
Wang, G.; Yin, W.; Zhou, Z.Y.; Hsieh, K.L.; Liu, J.K. New iridoids from the fruits of Crescentia cujete. J. Asian Nat. Prod. Res., 2010, 12(9), 770-775.
[http://dx.doi.org/10.1080/10286020.2010.503189] [PMID: 20839124]

Rights & Permissions Print Cite
Article Metrics
21
1
© 2024 Bentham Science Publishers | Privacy Policy