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Research Article

伊立肽素对乳腺癌的抗肿瘤作用与雌激素受体有关

卷 21, 期 1, 2021

发表于: 30 May, 2020

页: [73 - 85] 页: 13

弟呕挨: 10.2174/1566524020666200530212440

价格: $65

摘要

目的：我们研究伊卡利汀对乳腺癌的抗癌作用及其机制。 材料和方法：均使用雌激素受体（ER）阳性乳腺癌细胞MCF-7和ER阴性MDA-MB-231细胞。我们通过伤口愈合测定法和transwell测定法检查了icaritin对增殖和迁移的影响。流式细胞仪分析了MCF-7和MDA-MB-231细胞的细胞凋亡和细胞周期。通过蛋白质印迹，a啶橙染色和共聚焦显微镜评估MCF-7和MDA-MB-231细胞的细胞自噬。我们还通过蛋白质印迹检测了凋亡相关基因的表达。另外，使用自噬抑制剂来研究是否诱导了细胞保护性自噬。同时，利用ER抑制剂来研究ER是否参与自噬。 结果：伊立肽抑制了MDA-MB-231和MCF-7细胞的增殖和迁移，并诱导了其细胞周期停滞。伊立肽通过激活caspase-3显着诱导MDA-MB-231细胞凋亡。 icaritin刺激MCF-7细胞自噬，如LC3II / LC3I增加，p62降解增强，内源性LC3泪点形成的积累和自噬通量增加所证明的。伊立肽通过上调AMPK和ULK1的磷酸化诱导自噬。氯喹是一种自噬抑制剂，可增加依卡替丁诱导的MCF-7细胞凋亡和增殖抑制。同时，ER抑制剂他莫昔芬逆转了依卡瑞汀诱导的MCF-7细胞自噬和增殖抑制。 结论：我们的研究表明，icaritin对乳腺癌的抗肿瘤作用与ER有关，这表明icaritin的临床应用应考虑ER的状况。

关键词: 伊立肽，雌激素受体（ER），自噬，细胞凋亡，AMPK，ULK1。

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