Abstract
Quantification of lipids can be broadly classified into the following methods:
gravimetric analysis, which employs the principle of phase separation, the acid value is
based on the property of rancidity of fats, saponification value which relies on alkali
required to saponify (hydrolyze) lipids containing mixture, iodine value depends on the
interaction of fats with halogen iodine, and blood cholesterol estimation determines the
cholesterol level by cholesterol esterase enzyme-based assay. These assays have been
explained in detail in this chapter. Advanced techniques used for lipid quantification
utilize the principles of chromatography (Thin-layer chromatography, gas
chromatography, and HPLC) and spectroscopy (infrared spectroscopy, Raman
spectroscopy, nuclear magnetic resistance spectroscopy, and fluorescence
spectroscopy), and different colourimetric assays are discussed in detail in this chapter.
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