(Review Article) Screening for Disease-Markers and Investigating Drug Effects by Proteome Profiling: Can it Meet Expectations?

Christopher      Gerner

doi:10.2174/138620704772884779

Abstract

Drugs exert their functions mainly by affecting proteins. Therefore, it seems straightforward to focus on proteins in order to investigate drug effects. Unfortunately, proteins are of very high complexity, rendering it much more difficult to screen for protein alterations as compared to gene regulation. However, the efficiencyand applicability of proteome analysis has been dramatically increased recently. We are on the way to be ableto comprehensively assess disease-related proteome alterations, which may become an essential source ofinformation for knowledge-based drug design. This review will provide an overview of current techniques inproteome analysis, focusing on screening technologies for biomedical research. An outlook at the futurepotential of proteomics supported by modern bioinformatics will highlight why proteomics is worth the effort.

Keywords: 2d-page, autoradiography, fluorography, mass spectrometry, mudpit, quantitative, proteomics