选定的委陵菜属植物水提取物对人正常结肠细胞生物活性的影响

Title:Aqueous Extracts of Selected Potentilla Species Modulate Biological Activity of Human Normal Colon Cells

Volume: 16 Issue: 13

Author(s): Roman Paduch, Adrian Wiater, Marcello Locatelli, Malgorzata Pleszczyńska and Michal Tomczyk

Affiliation:

关键词: 细胞毒性，正常人结肠癌细胞，委陵菜属，蔷薇科

摘要: Potentilla L. (Rosaceae) species have been used in traditional and in folk medicine for many years. This study characterized the activity of extracts from aerial parts of selected Potentilla species: P. argentea, P. anserina, P. grandiflora and P. erecta as well as one species of closely related to the genus Potentilla, Drymocallis rupestris (syn. P. rupestris). The biological activities were analyzed using MTT, NR and DPPH assays on CCD 841 CoTr and CCD-18Co cells. Moreover, cell morphology and cytoskeletal actin F-filaments organization and IL-6 and IL-10 levels by ELISA were analyzed after 24 h of incubation. Potentilla extracts at dose levels between 25 and 250 µg/mL were analyzed. For ELISA, 15 µg/mL and 30 μg/mL were chosen. When mitochondrial succinyl dehydrogenase activity was tested (MTT assay) only extract obtained from P. erecta at lower concentrations (up to 125 µg/mL) suppressed metabolism of myofibroblasts, while epithelial cells mitochondrial enzyme activity increased after incubation with all extracts. In Neutral Red (NR) method cellular membrane disturbance of both cell cultures was found after D. rupestris and P. grandiflora addition. Moreover, strong influence on epithelial cells was also found for P. anserina. All extracts showed similar, concentration-dependent free radical scavenging (DPPH) effect. Potentilla extracts, especially at lower concentration, decreased IL-6 production in myofibroblasts but the level of the cytokine was found to be stable in epithelial cells. IL-10 analysis revealed that P. argentea, D. rupestris, P. erecta extracts decrease cytokine level in myofibroblasts, while only when higher concentration were applied, decreased cytokine level produced by epithelial cells was found. F-actin filaments staining revealed that Potentilla extracts significantly influence on cellular cytoskeleton organization. Potentilla extracts influence on cells of human colon wall lining modulating the main features of them (viability, cytokine production). Moreover, as a free radical reducing agents, may be successfully used in the prevention of colon disorders.

Cite this article as:

Roman Paduch, Adrian Wiater, Marcello Locatelli, Malgorzata Pleszczyńska and Michal Tomczyk , Aqueous Extracts of Selected Potentilla Species Modulate Biological Activity of Human Normal Colon Cells, Current Drug Targets 2015; 16 (13) . https://dx.doi.org/10.2174/1389450116666141205160444