BSA Au Clusters as a Probe for Enhanced Fluorescence Detection Using Multipulse Excitation Scheme

Title:BSA Au Clusters as a Probe for Enhanced Fluorescence Detection Using Multipulse Excitation Scheme

Volume: 14 Issue: 13

Author(s): Sangram L. Raut, Ryan Rich, Rafal Fudala, R. Kokate, J.D. Kimball, Julian Borejdo, Jamboor K. Vishwanatha, Zygmunt Gryczynski and Ignacy Gryczynski

Affiliation:

Keywords: Autofluorescence, BSA Au clusters, long lifetime, multipulse excitation, signal to noise.

Abstract: Although BSA Au clusters fluoresce in red region (λ_max: 650nm), they are of limited use due to low fluorescence quantum yield (~6%). Here we report an enhanced fluorescence imaging application of fluorescent bio-nano probe BSA Au clusters using multipulse excitation scheme. Multipulse excitation takes advantage of long fluorescence lifetime (> 1µs) of BSA Au clusters and enhances its fluorescence intensity 15 times over short lived cellular auto-fluorescence. Moreover we have also shown that by using time gated detection strategy signal (fluorescence of BSA Au clusters) to noise (auto-fluorescence) ratio can be increased by 30 fold. Thereby with multipulse excitation long lifetime probes can be used to develop biochemical assays and perform optical imaging with zero background.

Cite this article as:

Raut L. Sangram, Rich Ryan, Fudala Rafal, Kokate R., Kimball J.D., Borejdo Julian, Vishwanatha K. Jamboor, Gryczynski Zygmunt and Gryczynski Ignacy, BSA Au Clusters as a Probe for Enhanced Fluorescence Detection Using Multipulse Excitation Scheme, Current Pharmaceutical Biotechnology 2013; 14 (13) . https://dx.doi.org/10.2174/1389201015666140523161038