Selection and Characterization of Human Anti-MAGE-A1 scFv and Immunotoxin

Title:Selection and Characterization of Human Anti-MAGE-A1 scFv and Immunotoxin

Volume: 13 Issue: 8

Author(s): Hong Lin, Yuan Mao, Da-Wei Zhang, Hu Li, Jin-Rong Qiu, Jin Zhu and Ren-Jie Chen

Affiliation:

Keywords: Phage display antibody library, anti-MAGE-A1 scFv fragment, immunotoxin.

Abstract: Melanoma-associated antigen (MAGE) is expressed on the surface of multiple tumor cell types and is a promising target of biotherapeutic drug delivery via the anti-MAGE-A1 antibody. In this study, a human single-chain variable fragment (scFv) antibody phage library was generated and applied to recombinant MAGE-A1-coated immunotubes by phage display technology. The soluble anti- MAGE-A1 scFv was expressed and purified by immobilized metal-chelated affinity chromatography (IMAC). The anti-MAGE-A1 scFv could bind native MAGE-A1 confirmed by enzyme-linked immunosorbent assay (ELISA), dot blot, and immunoprecipitation (IP) analysis. The immunotoxin was expressed and purified by IMAC successfully. The results indicated that the human anti-MAGE-A1 immunotoxin could provide a valuable drug for clinic cancer therapy.

Cite this article as:

Lin Hong, Mao Yuan, Zhang Da-Wei, Li Hu, Qiu Jin-Rong, Zhu Jin and Chen Ren-Jie, Selection and Characterization of Human Anti-MAGE-A1 scFv and Immunotoxin, Anti-Cancer Agents in Medicinal Chemistry 2013; 13 (8) . https://dx.doi.org/10.2174/18715206113139990134