Abstract
Biotransformation of (+)-α-pinene to (+)-verbenone using shaking cultures of Cladosporium cladosporides (MTCC 1003) was investigated. Cladosporium cladosporides showed specific transformation of (+)-α-pinene with 86.8±3.0% conversion (relative integrated area gas chromatography–mass spectrometry (GC-MS)) of (+)-verbenone in 7 day incubation. The amount of verbenone formed was observed at 1, 3, 5 and 7th day where it is gradually increased. Verbenol was formed as intermediate product and slowly biocatalyzed to verbenone.
Keywords: Biotransformation, Cladosporium cladosporides, Verbenone, Oxidation, GC-MS
Current Catalysis
Title:Microbial Transformation of (+)-α-Pinene to (+)-Verbenone by Resting Cell Suspension of Cladosporium Cladosporides
Volume: 1 Issue: 2
Author(s): Siddhardha Busi, Ramanjaneyulu GS and Prabhakar S
Affiliation:
Keywords: Biotransformation, Cladosporium cladosporides, Verbenone, Oxidation, GC-MS
Abstract: Biotransformation of (+)-α-pinene to (+)-verbenone using shaking cultures of Cladosporium cladosporides (MTCC 1003) was investigated. Cladosporium cladosporides showed specific transformation of (+)-α-pinene with 86.8±3.0% conversion (relative integrated area gas chromatography–mass spectrometry (GC-MS)) of (+)-verbenone in 7 day incubation. The amount of verbenone formed was observed at 1, 3, 5 and 7th day where it is gradually increased. Verbenol was formed as intermediate product and slowly biocatalyzed to verbenone.
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Cite this article as:
Busi Siddhardha, GS Ramanjaneyulu and S Prabhakar, Microbial Transformation of (+)-α-Pinene to (+)-Verbenone by Resting Cell Suspension of Cladosporium Cladosporides, Current Catalysis 2012; 1 (2) . https://dx.doi.org/10.2174/2211544711201020129
DOI https://dx.doi.org/10.2174/2211544711201020129 |
Print ISSN 2211-5447 |
Publisher Name Bentham Science Publisher |
Online ISSN 2211-5455 |
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