Abstract
Electropermeabilization is one of the non-viral methods successfully used to transfer genes into living cells invitro and in-vivo. Although this method shows promises in the field of gene therapy, very little is known about the basic processes supporting plasmid DNA transfer. In contrast to small molecules that have direct access to the cytoplasm, plasmid DNA forms a long-lasting complex with the permeabilized membrane. The present letter reports the semi-quantitative analysis of the effect of electric pulse intensity on plasmid DNA/membrane interaction at the single-cell level using fluorescence microscopy. The raising of electric field intensity induces an increase in the cellular perimeter of plasmid DNA/membrane interaction and in the total number of plasmid DNA complexes. The increase in the perimeter of plasmid DNA/membrane interaction is in good agreement with the theoretical perimeter of membrane permeabilization. This theory easily explains the quantitative increase in plasmid DNA and gives explanations for the success of electro-mediated gene delivery.
Keywords: Electropermeabilization, gene delivery, pDNA/membrane interaction, electric field intensity, plasmid DNA, immobilization, pDNA Staining
Drug Delivery Letters
Title:Effect of Electric Field Intensity on Plasmid DNA/Membrane Interaction during In-Vitro Gene Electrotransfer
Volume: 2 Issue: 1
Author(s): Jean-Michel Escoffre, Elisabeth Bellard, Emilie Phez, Marie-Pierre Rols and Cyril Favard
Affiliation:
Keywords: Electropermeabilization, gene delivery, pDNA/membrane interaction, electric field intensity, plasmid DNA, immobilization, pDNA Staining
Abstract: Electropermeabilization is one of the non-viral methods successfully used to transfer genes into living cells invitro and in-vivo. Although this method shows promises in the field of gene therapy, very little is known about the basic processes supporting plasmid DNA transfer. In contrast to small molecules that have direct access to the cytoplasm, plasmid DNA forms a long-lasting complex with the permeabilized membrane. The present letter reports the semi-quantitative analysis of the effect of electric pulse intensity on plasmid DNA/membrane interaction at the single-cell level using fluorescence microscopy. The raising of electric field intensity induces an increase in the cellular perimeter of plasmid DNA/membrane interaction and in the total number of plasmid DNA complexes. The increase in the perimeter of plasmid DNA/membrane interaction is in good agreement with the theoretical perimeter of membrane permeabilization. This theory easily explains the quantitative increase in plasmid DNA and gives explanations for the success of electro-mediated gene delivery.
Export Options
About this article
Cite this article as:
Escoffre Jean-Michel, Bellard Elisabeth, Phez Emilie, Rols Marie-Pierre and Favard Cyril, Effect of Electric Field Intensity on Plasmid DNA/Membrane Interaction during In-Vitro Gene Electrotransfer, Drug Delivery Letters 2012; 2 (1) . https://dx.doi.org/10.2174/2210304x11202010022
DOI https://dx.doi.org/10.2174/2210304x11202010022 |
Print ISSN 2210-3031 |
Publisher Name Bentham Science Publisher |
Online ISSN 2210-304X |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Dietary Polyphenols for Treatment of Alzheimer’s Disease– Future Research and Development
Current Pharmaceutical Biotechnology Two Handy Geometric Prediction Methods of Cancer Growth
Current Medical Imaging Endocrine Disruptor Agent Nonyl Phenol Exerts An Estrogen-like Transcriptional Activity on Estrogen Receptor Positive Breast Cancer Cells
Current Medicinal Chemistry Modern Drug Discovery Technologies: Opportunities and Challenges in Lead Discovery
Combinatorial Chemistry & High Throughput Screening EDITORIAL: Advances in Developing Versatile Tools for the Discovery of Novel Therapeutics
Current Topics in Medicinal Chemistry Advances in Photodynamic Therapy Assisted by Electroporation
Current Drug Metabolism Therapeutic Monoclonal Antibodies: Strategies and Challenges for Biosimilars Development
Current Medicinal Chemistry Pharmacological Intervention of Cyclooxygenase-2 and 5-Lipoxygenase Pathways. Impact on Inflammation and Cancer
Current Pharmaceutical Design Novel Insights Into c-Src
Current Pharmaceutical Design Bothrops pauloensis Snake Venom Toxins: The Search for New Therapeutic Models
Current Topics in Medicinal Chemistry Antimicrobial Peptides: Mediators of Innate Immunity as Templates for the Development of Novel Anti-Infective and Immune Therapeutics
Current Pharmaceutical Design Characterization of Mitogen-Activated Protein Kinase Expression in Nucleus Accumbens and Hippocampus of Rats Subjected to Food Selection in the Cafeteria Diet Protocol
CNS & Neurological Disorders - Drug Targets Blocking the PI3K/PKB Pathway in Tumor Cells
Current Medicinal Chemistry - Anti-Cancer Agents Mitochondria-Targeted Antioxidant Peptide SS31 Protects the Retinas of Diabetic Rats
Current Molecular Medicine Dietary Phytochemicals in Chemoprevention of Cancer: An Update
Immunology, Endocrine & Metabolic Agents in Medicinal Chemistry (Discontinued) Role of mTOR Signaling in Tumor Cell Motility, Invasion and Metastasis
Current Protein & Peptide Science Immune Response to Helper Dependent Adenoviral Mediated Liver Gene Therapy: Challenges and Prospects
Current Gene Therapy Medical Image Examination using Traditional and Soft-computing Approaches
Current Medical Imaging The Scatter Factor Signaling Pathways as Therapeutic Associated Target in Cancer Treatment
Current Medicinal Chemistry Estrogen Receptor Polymorphisms: Significance to Human Physiology, Disease and Therapy
Recent Patents on DNA & Gene Sequences