Hydrophilic Interaction Liquid Chromatography-Tandem Mass Spectrometry for Drug Development

Title: Hydrophilic Interaction Liquid Chromatography-Tandem Mass Spectrometry for Drug Development

Volume: 7 Issue: 3

Author(s): Andrea L.O.Hebb, Virender Bhan, Alexander D. Wishart, Craig S. Moore, George S. Robertson and Yunsheng Hsieh

Affiliation:

Keywords: Hydrophilic interaction chromatography, tandem mass spectrometry, pharmaceutical analysis, drug development, HILIC, mass spectrometric (MS/MS) methods, HPLC, electrospray ionization, ESI, atmospheric pressure, APCI, atmospheric pressure pho-toionization, APPI, Re-versed-phase liquid chromatography, Ultrahigh-pressure liquid chromatography, liquid, –, liquid extraction, trifluoroacetic acid, solid-phase extraction, SPE, pro-tein precipitation, PPT, ZWITTERIONIC HILIC-MS/MS, ZIC, AMINO-MODE HILIC-MS/MS, CAPILLARY HILIC-MS/MS, GABA, ULTRA-PERFORMANCE HILIC-MS/MS, Caco-2 assay, phosphatidylcholine, lysophosphatidylcholine

Abstract: Hydrophilic interaction chromatography (HILIC) offers a difference in selectivity as compared to traditional normal phase and reversed-phase liquid chromatography and therefore has great potential for the separation of a variety of pharmaceuticals. This review is devoted to summarizing HILIC coupled to tandem mass spectrometric (MS/MS) methods for the rapid, sensitive and accurate determinations of small molecules employed for supporting drug discovery. The perspectives and challenges on performing HILIC-MS/MS assays are also presented.

Cite this article as:

L.O.Hebb Andrea, Bhan Virender, Wishart D. Alexander, Moore S. Craig, Robertson S. George and Hsieh Yunsheng, Hydrophilic Interaction Liquid Chromatography-Tandem Mass Spectrometry for Drug Development, Current Drug Discovery Technologies 2010; 7 (3) . https://dx.doi.org/10.2174/157016310793180521