Adsorption and Bioactivity of Tyrosine Hydroxylase on Gold Surfaces and Nanoparticles

Title: Adsorption and Bioactivity of Tyrosine Hydroxylase on Gold Surfaces and Nanoparticles

Volume: 17 Issue: 11

Author(s): Oyvind Halskau, Sondre Volden, Ana C. Calvo, Aurora Martinez and Wilhelm R. Glomm

Affiliation:

Keywords: Gold nanoparticles, recombinant human tyrosine hydroxylase isoform 1 (hTH1), adsorption, stability, enzymatic activity, Parkinson's disease, nanoparticles, recombinant, enzymatic, Tyrosine Hydroxylase, (L-DOPA), (MUA), QCM-Z500, quartz crystals, Citrate, pKa, pH, electrostatic, kinetic, L-DOPA, TRANSIL, D-fplots, QCM

Abstract: Tyrosine hydroxylase is studied in terms of adsorption behaviour on gold surfaces and various passivating layers. Results reveal differences in layer formation, where mercaptoundecanoic acid-coated gold shows the best potential in terms of adsorbed mass. Nanoparticles with this coating are subsequently tested for enzymatic activity, which remains at attenuated levels.

Cite this article as:

Halskau Oyvind, Volden Sondre, C. Calvo Ana, Martinez Aurora and R. Glomm Wilhelm, Adsorption and Bioactivity of Tyrosine Hydroxylase on Gold Surfaces and Nanoparticles, Protein & Peptide Letters 2010; 17 (11) . https://dx.doi.org/10.2174/0929866511009011376