The Inhibitory Effect of Propofol on Bovine Lactoperoxidase

Title: The Inhibitory Effect of Propofol on Bovine Lactoperoxidase

Volume: 16 Issue: 1

Author(s): Melda Sisecioglu, Murat Cankaya, Ilhami Gulcin and Hasan Ozdemir

Affiliation:

Keywords: Propofol, lactoperoxidase, enzyme, purification, inhibition, chromatography

Abstract: Propofol (2,6-diisopropylphenol) is a hypnotic intravenous agent with in vivo antioxidant properties. This study was undertaken to examine the in vitro effect of propofol on lactoperoxidase (LPO; E.C. 1.11.1.7) obtained from bovine milk. Lactoperoxidase was purified with three purification steps: Amberlite CG-50 resin, CM-Sephadex C-50 ionexchange chromatography and Sephadex G-100 gel filtration chromatography, respectively. Lactoperoxidase was purified with a yield of 21.6%, a specific activity of 34 EU/mg proteins and 14.7-fold purification. One enzyme unit is defined as the oxidation of 1 μmol ABTS per min under the assay condition (25oC, pH: 6.0). To determine enzyme purity, sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed and single band was observed. The effect of propofol on lactoperoxidase were determined using 2,2-azino-bis (3-ethylbenzthiazoline-6 sulfonic acid) diammonium salt (ABTS) as a chromogenic substrate. The IC50 value of propofol was found as 15.97 μM. Also, Ki constant for propofol was 3.72 μM and propofol was found as competitive inhibitor.

Cite this article as:

Sisecioglu Melda, Cankaya Murat, Gulcin Ilhami and Ozdemir Hasan, The Inhibitory Effect of Propofol on Bovine Lactoperoxidase, Protein & Peptide Letters 2009; 16 (1) . https://dx.doi.org/10.2174/092986609787049394