Synthesis and Cellular Uptake of Fluorescently Labeled 1-Chloromethyl-5- Hydroxy-1, 2-Dihydro-3H-Benz [e] Indole (Seco-CBI)- Pyrrole Polyamide Conjugates

Rohtash      Kumar; Dinesh      Rai; Sandra   L.   Marcus; Simon   Ching   Chun Ko; J.   William   Lown

doi:10.2174/1570178043488383

Abstract

The synthesis is described of 1-chloromethyl-5-hydroxy-1, 2-dihydro-3H-benz [e] indole (seco- CBI)- pyrrole polyamide conjugates (1-4) which were labeled with a fluorescent Fmoc moiety to evaluate their cellular uptake. Confocal laser scanning microscopy (CLSM) indicates that these DNA minor groove-binding agents localize in the cytoplasm and organelles.

Keywords: 1-chloromethyl-5-, hydroxy-1, 2-dihydro-3h-benz [e] indole, pyrrole polyamide conjugates, fluorescent fmoc, groove-binding agents

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Letters in Organic Chemistry

Title: Synthesis and Cellular Uptake of Fluorescently Labeled 1-Chloromethyl-5- Hydroxy-1, 2-Dihydro-3H-Benz [e] Indole (Seco-CBI)- Pyrrole Polyamide Conjugates

Volume: 1 Issue: 2

Author(s): Rohtash Kumar, Dinesh Rai, Sandra L. Marcus, Simon Ching Chun Ko and J. William Lown

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Keywords: 1-chloromethyl-5-, hydroxy-1, 2-dihydro-3h-benz [e] indole, pyrrole polyamide conjugates, fluorescent fmoc, groove-binding agents

Abstract: The synthesis is described of 1-chloromethyl-5-hydroxy-1, 2-dihydro-3H-benz [e] indole (seco- CBI)- pyrrole polyamide conjugates (1-4) which were labeled with a fluorescent Fmoc moiety to evaluate their cellular uptake. Confocal laser scanning microscopy (CLSM) indicates that these DNA minor groove-binding agents localize in the cytoplasm and organelles.

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Kumar Rohtash, Rai Dinesh, Marcus L. Sandra, Chun Ko Ching Simon and Lown William J., Synthesis and Cellular Uptake of Fluorescently Labeled 1-Chloromethyl-5- Hydroxy-1, 2-Dihydro-3H-Benz [e] Indole (Seco-CBI)- Pyrrole Polyamide Conjugates, Letters in Organic Chemistry 2004; 1 (2) . https://dx.doi.org/10.2174/1570178043488383