Effect of Pressure on Nucleotide Binding to Yeast Alcohol Dehydrogenase

Title: Effect of Pressure on Nucleotide Binding to Yeast Alcohol Dehydrogenase

Volume: 12 Issue: 5

Author(s): Gene Kidman and Dexter B. Northrop

Affiliation:

Keywords: hypsochromic shift, bathochromic shift, hydrostatic pressure, yeast alcohol dehydrogenase, nadh, cycloamylose

Abstract: Binding of reduced nicotinamide adenine dinucleotide to yeast alcohol dehydrogenase results in a hypsochromic shift of its absorbance maximum at 340 nm. Application of high hydrostatic pressure to the enzymenucleotide complex returns the absorbance maximum to longer wavelengths. This pressure-dependent bathochromic shift validates one of two assignments on the effects of pressure on the kinetics of the enzymatic oxidation of benzyl alcohol, namely the protein-ligand conformational change of the capturing form of enzyme.

Cite this article as:

Kidman Gene and Northrop B. Dexter, Effect of Pressure on Nucleotide Binding to Yeast Alcohol Dehydrogenase, Protein & Peptide Letters 2005; 12 (5) . https://dx.doi.org/10.2174/0929866054395293