Overexpression and Purification of Recombinant Eel Calcitonin and Its Phylogenetic Analysis

Title: Overexpression and Purification of Recombinant Eel Calcitonin and Its Phylogenetic Analysis

Volume: 12 Issue: 3

Author(s): Chiranjib Chakraborty, Shyam Sundar Nandi, Biplab Sarkar and S. Sinha

Affiliation:

Keywords: recombinant eel calcitonin (ect), amidating enzyme, therapeutic protein, pchic9

Abstract: Calcitonin (CT), a peptide hormone that is widely used for the treatment of osteoporosis, Pagets disease, hypercalcemic shock and chronic pain in terminal cancer patients, is produced by the para-follicular cells of the thyroid gland in mammals and by the ultimobranchial gland of birds and fish. Fish calcitonin, like eel calcitonin (eCT), is more potent and longer lasting than human CT and is one of the many bioactive peptides that require C-terminal amidation for full biological activity. In this study we describe the over-expression and over-production of C-terminal amidated eCT in recombinant Streptomyces avermitilis. A phylogenetic analysis was performed with all the known CT amino acid sequences.

Cite this article as:

Chakraborty Chiranjib, Nandi Sundar Shyam, Sarkar Biplab and Sinha S., Overexpression and Purification of Recombinant Eel Calcitonin and Its Phylogenetic Analysis, Protein & Peptide Letters 2005; 12 (3) . https://dx.doi.org/10.2174/0929866053587147