Production of Minichaperone (sht GroEL191-345) and Its Function in the Refolding of Recombinant Human Interferon Gamma

Title: Production of Minichaperone (sht GroEL191-345) and Its Function in the Refolding of Recombinant Human Interferon Gamma

Volume: 12 Issue: 1

Author(s): Yi-Xin Guan, Zheng-Zheng Fei, Man Luo, Shan-Jing Yao and Man-Gi Cho

Affiliation:

Keywords: minichaperone, sht groel191-345, cultivation, purification, refolding, recombinant human interferon gamma

Abstract: The recombinant minichaperone sht GroEL191-345 was cultivated in a 3.7 L stirred bioreactor with the high yield of 216.2 mg / L broth. In the refolding of recombinant human interferon gamma (rhuIFN-γ) inclusion bodies, more than 2-3 fold enhancement in protein mass recovery and total activity were observed in the presence of free or immobilized minichaperone to the refolding buffer.

Cite this article as:

Guan Yi-Xin, Fei Zheng-Zheng, Luo Man, Yao Shan-Jing and Cho Man-Gi, Production of Minichaperone (sht GroEL191-345) and Its Function in the Refolding of Recombinant Human Interferon Gamma, Protein & Peptide Letters 2005; 12 (1) . https://dx.doi.org/10.2174/0929866053405995