A Surface Immobilization Method of Endoglucanase from Cellulomonas biazotea Mutant Improved Catalytic Properties of Biocatalyst During Processing

Title: A Surface Immobilization Method of Endoglucanase from Cellulomonas biazotea Mutant Improved Catalytic Properties of Biocatalyst During Processing

Volume: 14 Issue: 7

Author(s): Muhammad Ibrahim Rajoka, Yasmin Zia and Khalil-ur-Rehman

Affiliation:

Keywords: Eudragit L-100, immobilization kinetics, melting point, substrate binding energy, thermodynamics, transition state stabilization

Abstract: Purified endoglucanase from C. biazotea mutant 51SMr was successfully immobilized on Eudragit L-100, with 75 % yield of immobilization. This method improved the kinetic and thermodynamic properties of the enzyme. Immobilization significantly decreased entropy and enthalpy of inactivation of biocatalyst and made it functionally and thermodynamically more stable and reusable compared to free one.

Cite this article as:

Muhammad Ibrahim Rajoka , Yasmin Zia and Khalil-ur-Rehman , A Surface Immobilization Method of Endoglucanase from Cellulomonas biazotea Mutant Improved Catalytic Properties of Biocatalyst During Processing, Protein & Peptide Letters 2007; 14 (7) . https://dx.doi.org/10.2174/092986607781483886