Crystallization and Preliminary Crystallographic Study of DNA Polymerase from Pyrococcus furiosus

Title: Crystallization and Preliminary Crystallographic Study of DNA Polymerase from Pyrococcus furiosus

Volume: 14 Issue: 4

Author(s): Hirokazu Nishida, Maiko Tanabe, Yoshizumi Ishino, Takuji Oyama and Kosuke Morikawa

Affiliation:

Keywords: DNA polymerase, crystallization, archaea

Abstract: A new member of archaeal DNA polymerase from Pyrococcus furiosus was crystallized. Diffraction data to 3.1 Å of the selenomethionine-derivatized crystal were collected, and preliminary crystallographic study has been completed. The crystal belongs to the space group C2 with unit cell parameters of a = 93.2 Å, b = 124.9 Å, c = 87.7 Å, α = 90 , β = 109.7® , and γ = 90 . Assuming the presence of one molecule in the asymmetric unit, the solvent content of the crystal is estimated to be 54%, corresponding to a Matthews coefficient VM of 2.7Å3 Da-1.

Cite this article as:

Nishida Hirokazu, Tanabe Maiko, Ishino Yoshizumi, Oyama Takuji and Morikawa Kosuke, Crystallization and Preliminary Crystallographic Study of DNA Polymerase from Pyrococcus furiosus, Protein & Peptide Letters 2007; 14 (4) . https://dx.doi.org/10.2174/092986607780363853