Abstract
Medical sensing systems using isolated or intact glutamate receptor (GluR) ion channels and glutamate oxidase (GluOx) are discussed for L-glutamate, one of the principal neurotransmitter in the central nervous systems of mammalian brain, and related agonists. The GluR-based sensing system used for the evaluation of signal transduction ability of GluR channels demonstrate that the agonist selectivity based on the signal transduction ability is not parallel to that of the binding assay. On the other hand, the appropriate design of the enzyme system, namely glutamate oxidase (GluOx), in combination with horseradish peroxidase (HRP), enables to real-time monitoring of L-glutamate in vivo and in vitro and also to visualize its release in submerged, acute mouse hippocampal slices.
Keywords: Glutamate receptor ion channels, Glutamate oxidase, acute brain slice, gonist selectivity, patch sensor, visualization of glutamate release, micro sensor, agonist selectivity
Mini-Reviews in Medicinal Chemistry
Title: Receptors and Enzymes for Medical Sensing of L-Glutamate
Volume: 6 Issue: 10
Author(s): Ayumi Hirano and Masao Sugawara
Affiliation:
Keywords: Glutamate receptor ion channels, Glutamate oxidase, acute brain slice, gonist selectivity, patch sensor, visualization of glutamate release, micro sensor, agonist selectivity
Abstract: Medical sensing systems using isolated or intact glutamate receptor (GluR) ion channels and glutamate oxidase (GluOx) are discussed for L-glutamate, one of the principal neurotransmitter in the central nervous systems of mammalian brain, and related agonists. The GluR-based sensing system used for the evaluation of signal transduction ability of GluR channels demonstrate that the agonist selectivity based on the signal transduction ability is not parallel to that of the binding assay. On the other hand, the appropriate design of the enzyme system, namely glutamate oxidase (GluOx), in combination with horseradish peroxidase (HRP), enables to real-time monitoring of L-glutamate in vivo and in vitro and also to visualize its release in submerged, acute mouse hippocampal slices.
Export Options
About this article
Cite this article as:
Hirano Ayumi and Sugawara Masao, Receptors and Enzymes for Medical Sensing of L-Glutamate, Mini-Reviews in Medicinal Chemistry 2006; 6 (10) . https://dx.doi.org/10.2174/138955706778560102
DOI https://dx.doi.org/10.2174/138955706778560102 |
Print ISSN 1389-5575 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5607 |

- Author Guidelines
- Bentham Author Support Services (BASS)
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
The Challenges Involved in Modeling Toxicity Data In Silico: A Review
Current Pharmaceutical Design The Pathophysiology of CTGs and Types of Intrapartum Hypoxia
Current Women`s Health Reviews Review of Recent Patents on Foldable Ventricular Assist Devices
Recent Patents on Biomedical Engineering (Discontinued) Protective Effects of Diuretics Against the Development of Cardiovascular Disease in Patients with Chronic Kidney Disease: A Systematic Review
Cardiovascular & Hematological Agents in Medicinal Chemistry Cardiological Ultrasound Imaging
Current Pharmaceutical Design Genetics of Cholesterol and Lipoprotein Metabolism
Recent Patents on Cardiovascular Drug Discovery The Effects of Sevoflurane or Remifentanil on the Stress Response to Surgical Stimulus
Current Pharmaceutical Design Phenylbutyric Acid Protects Against Spatial Memory Deficits in a Model of Repeated Electroconvulsive Therapy
Current Neurovascular Research Viral Product Trafficking to Mitochondria, Mechanisms and Roles in Pathogenesis
Infectious Disorders - Drug Targets Obstetric Anaesthesia and Obesity
Current Women`s Health Reviews Role of Gap Junction Channel in the Development of Beat-to-Beat Action Potential Repolarization Variability and Arrhythmias
Current Pharmaceutical Design Pharmacotherapy of Abdominal Aortic Aneurysms
Current Vascular Pharmacology Fluid and Electrolyte Disorders in the Newborn: Sodium and Potassium
Current Pediatric Reviews Common Variants for Heart Failure
Current Genomics Peroxisome Proliferator-Activated Receptor (PPAR) in Metabolic Syndrome and Type 2 Diabetes Mellitus
Current Diabetes Reviews Personalized Medicine in Oncology: A Personal View with Myths and Facts
Current Clinical Pharmacology Clinical Pharmacotherapy and Drug Development for Pulmonary Arterial Hypertension
Recent Patents on Cardiovascular Drug Discovery Swimming Activity and Non-Protein Energy (NPE) Metabolism in Fish
Current Nutrition & Food Science Beta-Blockers in Pediatric Hypertrophic Cardiomyopathies
Reviews on Recent Clinical Trials Potential Targets for Intervention in Radiation-Induced Heart Disease
Current Drug Targets