Molecular Characterization of Antimicrobial Resistance and Virulence Genotyping among Helicobacter pylori-Positive Dyspeptic Patients in North Iran

Title:Molecular Characterization of Antimicrobial Resistance and Virulence Genotyping among Helicobacter pylori-Positive Dyspeptic Patients in North Iran

Volume: 24

Author(s): Ebrahim Kouhsari, Gholamreza Roshandel, Sara Hosseinzadeh, Sima Besharat, Vahid Khori and Taghi Amiriani*

Affiliation:

• Golestan Research Center of Gastroenterology and Hepatology, Golestan University of Medical Sciences, Gorgan, Iran

Abstract: Background: Iran has a relatively high prevalence of H. pylori, which correlates with high-risk areas for gastric cancer worldwide.

Methods: Our study aimed to investigate the underlying genetic mechanisms associated with resistance to metronidazole (frxA, rdxA), clarithromycin (23S rRNA), tetracycline (16S rRNA), and fluoroquinolone (gyrA) in H. pylori-positive dyspeptic patients using PCR and sequencing. We further examined the potential correlation between resistance profiles and various virulence genotypes.

Results: The rates of genetic mutations associated with resistance to metronidazole, fluoroquinolone, clarithromycin, and tetracycline were found to be 68%, 32.1%, 28.4%, and 11.1%, respectively. Well-documented multiple antibiotic resistance mutations were detected, such as rdxA and frxA (with missense and frameshift alterations), gyrA (Asp91, Asn87), 23S rRNA (A2142G, A2143G), and 16S rRNA (triple-base-pair substitutions AGA926-928→TTC). The cagA+ and vacA s1/m1 types were the predominant genotypes in our study. With the exception of metronidazole and tetracycline, no significant correlation was observed between the cagA+ and cagL+ genotypes and resistance-associated mutations.

Conclusion: The prevalence of antibiotic resistance-associated mutations in H. pylori was remarkably high in this region, particularly to metronidazole, ciprofloxacin, and clarithromycin. By conducting a simultaneous screening of virulence and resistance genotypes, clinicians can make informed decisions regarding the appropriate therapeutic regimen to prevent the escalation of antibiotic resistance against H. pylori infection in this specific geographical location.

Cite this article as:

Kouhsari Ebrahim, Roshandel Gholamreza, Hosseinzadeh Sara, Besharat Sima, Khori Vahid and Amiriani Taghi*, Molecular Characterization of Antimicrobial Resistance and Virulence Genotyping among Helicobacter pylori-Positive Dyspeptic Patients in North Iran, Infectious Disorders - Drug Targets 2024; 24 () : e090724231788 . https://dx.doi.org/10.2174/0118715265294927240617201332

DOI https://dx.doi.org/10.2174/0118715265294927240617201332	Print ISSN 1871-5265
Publisher Name Bentham Science Publisher	Online ISSN 2212-3989