Evaluation of Anti-Microbial Activity of Novel Polyherbal Lozenges for the Management of Infection

Kajal      Nagpal; Uditi      Handa; Anjali      Sharma; Deepshi      Arora; Fatimah      Jan

doi:10.2174/0122113525302223240606045531

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Abstract

Background: The use of commercial tinctures for the design of a formulation with improved anti-microbial activity in a polyherbal lozenge dosage form has not been described.

Objective: This study aimed to develop and evaluate the antimicrobial activity of a novel poly-herbal lozenge formulation containing Ashwagandha, Neem, and Tulsi tinctures in a single-dose administration. According to previous studies, each of these herbs could be used in herbal med-icine to provide relief from infection owing to their antimicrobial activity, besides other proper-ties, such as anticancer, antidiabetic, etc.

Method: The lozenges were prepared using the molding method using three concentrations (1, 3, and 5 % w/w) of commercialized herbal tinctures. Then, they were studied using different assays, including those designed to evaluate physical properties, stability, and antimicrobial ac-tivity.

Results: B4, containing 5% w/w of each tincture, showed the highest antimicrobial activity com-pared with the other batches. This batch exhibited the highest value of bacterial inhibition (17.1±0.07 mm), according to the agar well diffusion method, including Escherichia coli as a test microorganism. Hence, B4 was chosen for additional analyses, including physical properties and stability tests. The results followed Indian Pharmacopeia standards and ICH guidelines, re-spectively. The formulation was stable after 2 weeks, and no significant changes were observed in its physical properties or antimicrobial activity.

Conclusion: This study shows that polyherbal lozenges have anti-microbial activity, with a dis-integration period of 3.86±0.07 minutes in the B4 batch.

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DOI https://dx.doi.org/10.2174/0122113525302223240606045531	Print ISSN 2211-3525
Publisher Name Bentham Science Publisher	Online ISSN 2211-3533

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Evaluation of Anti-Microbial Activity of Novel Polyherbal Lozenges for the Management of Infection

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