Evaluation of the Antioxidant and Antimicrobial Properties of Hydrosol Extract and Essential Oil Derived from the Aerial Parts of Salvia argentea

Title:Evaluation of the Antioxidant and Antimicrobial Properties of Hydrosol Extract and Essential Oil Derived from the Aerial Parts of Salvia argentea

Volume: 22

Author(s): Ilyas Chikhi, Rachida Kerzabi, Safaa Nor el Houda Ziane, Fatima Benyoucef, Mohammed El Amine DIB*Alain Muselli

Affiliation:

• Laboratoire des Substances Naturelles et Bioactives (LASNABIO), Université Abou bekr Belkaid, Tlemcen, 13000 Algérie

Abstract:

Background: The increasing use of synthetic antimicrobials and antioxidants is a concern for human health.

Aims: The main objective of this study was to examine the chemical composition of the essential oil extracted from Salvia argentea and its hydrosol extract while evaluating their antioxidant and antimicrobial effects in vitro.

Materials and Methods: The essential oil was obtained by hydrodistillation, while the hydrosol extract was obtained by the liquid-liquid extraction method. The volatile compounds of the essential oil and hydrosol extract were analysed by gas chromatography (GC/FID) and gas chromatography coupled with mass spectrometry (GC/MS). Antioxidant activities were evaluated using two methods: free radical scavenging activity (DPPH) and iron reduction antioxidant power (FRAP), with BHT used as a positive control. The antimicrobial activity of the essential oil and hydrosol extract was evaluated using the agar disc diffusion method.

Results: According to the GC/FID and GC/MS analysis, the essential oil of S. argentea consisted mainly of germacrene D-4-ol (26.3%), epi-α-muurolol (19.2%), and epi-α-cadinol (10.2%). In contrast, the hydrosol extract consisted of oxygenated compounds, including (E)- phytol (23.5%), 14-hydroxy-δ-cadine (17.5%), caryophyllene oxide (11.5%), piperitone (13.5%), and piperitone oxide (11.5%). The essential oil and hydrosol extract showed positive antioxidant activity with both tests. It should be noted that the hydrosol extract showed the best performance, with an IC50 of 8.3 g/L with the DPPH and an IC50 of 12.3 g/L with the FRAP test compared to the BHT. The essential oil revealed antimicrobial activity with strong inhibition against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Bacillus subtilis compared to Gentamicin.

Conclusion: The results showed that essential oil and hydrosol extract have promising antimicrobial and antioxidant properties. These findings provide encouraging prospects for their potential use in the pharmaceutical or cosmetic sectors.

Cite this article as:

Chikhi Ilyas, Kerzabi Rachida, Ziane Nor el Houda Safaa, Benyoucef Fatima, DIB El Amine Mohammed*, Muselli Alain, Evaluation of the Antioxidant and Antimicrobial Properties of Hydrosol Extract and Essential Oil Derived from the Aerial Parts of Salvia argentea, Anti-Infective Agents 2024; 22 () : e030724231513 . https://dx.doi.org/10.2174/0122113525303193240606062041