Isolation, Characterization and Antibacterial Activity Study of Bioactive Compounds from Leaves of Cyphostemma cyphopetalum

Adem   Getahun   Mohammed; Mesfin   Getachew   Tadesse; Rakesh   Kumar   Bachheti; Yosef      Bayeh; Tesfay   G.   Ashebr

doi:10.2174/0122113525306978240520053843

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Abstract

Background: Cyphostemma cyphopetalum is a herbaceous climber species within the Vitaceae family, found in various tropical and subtropical regions of Africa. It has a history of traditional use in Ethiopia for treating various health issues in humans and livestock, includ-ing snake bites, rabies, and eczema. Despite its reported ethnomedicinal uses, there is a lack of sufficient scientific literature regarding the isolation and characterization of its bioactive chem-ical compounds.

Objective: The objective of this work was to isolate bioactive compounds from the leaves of Cyphostemma cyphopetalum, characterize their chemical structures using FTIR and NMR spec-troscopy, and evaluate their antimicrobial activities.

Methods: Isolation was done using column chromatographic separation techniques. Spectro-scopic techniques, including Fourier transform infrared and nuclear magnetic resonance, were used for functional group identification and structural elucidation. Moreover, the paper disc dif-fusion bioassay method was used to evaluate the antibacterial activity of the isolated bioactive compounds. Results: Three fractions were successfully isolated using chloroform with dichloromethane and chloroform with ethyl acetate as eluting solvents and silica gel as a stationary phase. After care-ful interpretation of the experimental results and comparison with the literature, the isolated fractions were constituted mainly by stigmasterol (fraction 1), β-sitosterol (fraction 2), and trans-resveratrol (fraction 3). The antibacterial activities of each isolated fraction were tested against Ralstonia solanacearum, a gram-negative bacterium, and Staphylococcus aureus, a gram-positive bacterial pathogen.

Comparatively, the highest mean inhibitory value of 10.03 ± 0.13 mm was exhibited by fraction 2, which was followed by 6.25±0.05 mm inhibition activity recorded for fraction 1 against R. solanacearum, while fraction 3 did not exhibit any bacterial activity against both the strains.

Conclusion: We have successfully isolated and characterized three compounds (1-3) from leaves of C. cyphopetalum and evaluated their antibacterial activity. A maximum mean inhibi-tory value of 10.03 ± 0.13 mm was exhibited by compound 2 against R. solanacearum.

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DOI https://dx.doi.org/10.2174/0122113525306978240520053843	Print ISSN 2211-3525
Publisher Name Bentham Science Publisher	Online ISSN 2211-3533

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Isolation, Characterization and Antibacterial Activity Study of Bioactive Compounds from Leaves of Cyphostemma cyphopetalum

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