Abstract
Background: Bacterial cellulose (BC) is a versatile biomaterial with numerous applications, and the identification of bacterial strains that produce it is of great importance. This study explores the effectiveness of a Stacked Autoencoder (SAE)-based deep learning method for the classification of bacterial cellulose-producing bacteria.
Objective: The primary objective of this research is to assess the potential of SAE-based classification models in accurately identifying and classifying bacterial cellulose-producing bacteria, with a particular focus on strain GZ-01.
Methods: Strain GZ-01 was isolated and subjected to a comprehensive characterization process, including morphological observations, physiological and biochemical analysis, and 16S rDNA sequencing. These methods were employed to determine the identity of strain GZ-01, ultimately recognized as Acetobacter Okinawa. The study compares the performance of SAE-based classification models to traditional methods like Principal Component Analysis (PCA).
Results: The SAE-based classifier exhibits outstanding performance, achieving an impressive accuracy of 94.9% in the recognition and classification of bacterial cellulose-producing bacteria. This approach surpasses the efficacy of conventional PCA in handling the complexities of this classification task.
Conclusion: The findings from this research highlight the immense potential of utilizing nanotechnology- driven data analysis methods, such as Stacked Autoencoders, in the realm of bacterial cellulose research. These advanced techniques offer a promising avenue for enhancing the efficiency and accuracy of bacterial cellulose-producing bacteria classification, which has significant implications for various applications in biotechnology and materials science.
Graphical Abstract
[http://dx.doi.org/10.1016/j.indcrop.2022.115147]
[http://dx.doi.org/10.1093/jimb/kuab071] [PMID: 34549273]
[http://dx.doi.org/10.1021/acssynbio.1c00290] [PMID: 34652130]
[http://dx.doi.org/10.4028/p-ex7xpa]
[http://dx.doi.org/10.1515/gps-2022-0038]
[http://dx.doi.org/10.1007/s00253-021-11107-2] [PMID: 33481067]
[http://dx.doi.org/10.1016/j.chemosphere.2021.132552] [PMID: 34648790]
[http://dx.doi.org/10.3923/pjbs.2021.612.617] [PMID: 34486336]
[http://dx.doi.org/10.1016/j.ijbiomac.2018.12.190] [PMID: 30593803]
[http://dx.doi.org/10.1016/j.fm.2021.103828] [PMID: 34119113]
[http://dx.doi.org/10.1016/j.jclepro.2021.126454]
[http://dx.doi.org/10.7717/peerj.11244] [PMID: 33976974]
[http://dx.doi.org/10.1016/j.ijbiomac.2021.08.157] [PMID: 34450146]
[http://dx.doi.org/10.1099/ijsem.0.005252] [PMID: 35175916]
[http://dx.doi.org/10.1016/j.carbpol.2021.118328] [PMID: 34364591]
[http://dx.doi.org/10.1073/pnas.2024015118] [PMID: 33729990]
[http://dx.doi.org/10.1109/LGRS.2022.3170702]
[http://dx.doi.org/10.1016/j.jcrc.2021.07.023] [PMID: 34438134]
[http://dx.doi.org/10.1016/j.ijid.2021.10.016]
[http://dx.doi.org/10.1016/j.btre.2021.e00660] [PMID: 34557388]
[http://dx.doi.org/10.1039/C4AN00801D]
[http://dx.doi.org/10.1016/j.heliyon.2023.e15482] [PMID: 37151686]
[http://dx.doi.org/10.1016/j.snb.2024.135645]
[http://dx.doi.org/10.1016/j.talanta.2023.124483] [PMID: 37019007]