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ISSN (Print): 1570-1808
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Research Article

Therapeutic Effects of Cortex Phellodendri, Epimedium brevicornu, and Earthworm on Chronic Prostatitis/Chronic Pelvic Pain Syndrome Mice

Author(s): Muhammad Naveed, Xingxing Cui, Jannat Bibi, Muhammad Furqan Akhtar, Muhammad Zia Ahmad, Abdullahi Ayuba, Muhammad Mohsin Khan, Asadullah Madni and Zhou Xiaohui*

Volume 21, Issue 15, 2024

Published on: 21 May, 2024

Page: [3298 - 3306] Pages: 9

DOI: 10.2174/0115701808240328231031103739

Price: $65

Abstract

Background: The use of traditional Chinese medicine (TCM) for the treatment of chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) is widespread. The purpose of this study is to investigate the preliminary mechanism of Epimedium brevicornu Maxim., Cortex Phellodendri Chinesis (CPC), and Earthworm in the CP/CPPS model.

Methods: Fifty C57BL/6 male mice were randomized into five groups: control, model, Epimedium brevicornu Maxim, Earthworm, and Cortex Phellodendri chinensis (CPC). To induce experimental autoimmune prostatitis (EAP), T2 peptide immune emulsion was subcutaneously injected into all groups except the control group on days 0 and 14 to induce experimental autoimmune prostatitis. Except for the control and model groups, all other groups were treated with Epimedium brevicornu Maxim., CPC, and earthworm after the successful induction of EAP.

Results: On days 1, 28, and 56, the micturition habits and pain sensitivity of rodents in each group were evaluated. Hematoxylin-eosin (H&E) staining was used to examine prostate inflammation, whereas enzyme-linked immunosorbent assay (ELISA) was used to measure the serum level of tumor necrosis factor-α (TNF). The Epimedium brevicornu Maxim. group significantly reduced the number of urine spots and frequency of pain response compared to the model group. Epimedium brevicornu Maxim. group and CPC group significantly reduced inflammatory cell infiltration and inflammatory lesions more than Earthworm groups. The serum levels of TNF-α were substantially reduced in the Epimedium brevicornu Maxim. and CPC groups compared to the model group.

Conclusion: Our findings showed that Epimedium brevicornu Maxim outperformed CPC and Earthworm for EAP treatment, which relieves urinary tract symptoms, enhances pain threshold, and lowers inflammation.

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[1]
Suskind, A.M.; Berry, S.H.; Ewing, B.A.; Elliott, M.N.; Suttorp, M.J.; Clemens, J.Q. The prevalence and overlap of interstitial cystitis/bladder pain syndrome and chronic prostatitis/chronic pelvic pain syndrome in men: Results of the RAND Interstitial Cystitis Epidemiology male study. J. Urol., 2013, 189(1), 141-145.
[http://dx.doi.org/10.1016/j.juro.2012.08.088] [PMID: 23164386]
[2]
Krieger, J.N.; Nyberg, L., Jr; Nickel, J.C. NIH consensus definition and classification of prostatitis. JAMA, 1999, 282(3), 236-237.
[http://dx.doi.org/10.1001/jama.282.3.236] [PMID: 10422990]
[3]
Polackwich, A.S.; Shoskes, D.A. Chronic prostatitis/chronic pelvic pain syndrome: A review of evaluation and therapy. Prostate Cancer Prostatic Dis., 2016, 19(2), 132-138.
[http://dx.doi.org/10.1038/pcan.2016.8] [PMID: 26951713]
[4]
Wenninger, K.; Heiman, J.R.; Rothman, I.; Berghuis, J.P.; Berger, R.E. Sickness impact of chronic nonbacterial prostatitis and its correlates. J. Urol., 1996, 155(3), 965-968.
[http://dx.doi.org/10.1016/S0022-5347(01)66359-6] [PMID: 8583619]
[5]
Liu, Y.; Mikrani, R. Chronic prostatitis/chronic pelvic pain syndrome and prostate cancer: Study of immune cells and cytokines. Fundam. Clin. Pharmacol., 2020, 34(2), 160-172.
[6]
Ihsan, A.U.; Khan, F.U.; Khongorzul, P.; Ahmad, K.A.; Naveed, M.; Yasmeen, S.; Cao, Y.; Taleb, A.; Maiti, R.; Akhter, F.; Liao, X.; Li, X.; Cheng, Y.; Khan, H.U.; Alam, K.; Zhou, X. Role of oxidative stress in pathology of chronic prostatitis/chronic pelvic pain syndrome and male infertility and antioxidants function in ameliorating oxidative stress. Biomed. Pharmacother., 2018, 106, 714-723.
[http://dx.doi.org/10.1016/j.biopha.2018.06.139] [PMID: 29990863]
[7]
Thakkinstian, A.; Attia, J.; Anothaisintawee, T.; Nickel, J.C. α‐blockers, antibiotics and anti‐inflammatories have a role in the management of chronic prostatitis/chronic pelvic pain syndrome. BJU Int., 2012, 110(7), 1014-1022.
[http://dx.doi.org/10.1111/j.1464-410X.2012.11088.x] [PMID: 22471591]
[8]
Appiya Santharam, M.; Khan, F.U.; Naveed, M.; Ali, U.; Ahsan, M.Z.; Khongorzul, P.; Shoaib, R.M.; Ihsan, A.U. Interventions to chronic prostatitis/Chronic pelvic pain syndrome treatment. Where are we standing and what’s next? Eur. J. Pharmacol., 2019, 857, 172429.
[http://dx.doi.org/10.1016/j.ejphar.2019.172429] [PMID: 31170381]
[9]
Zaidi, N.; Thomas, D.; Chughtai, B. Management of Chronic Prostatitis (CP). Curr. Urol. Rep., 2018, 19(11), 88.
[http://dx.doi.org/10.1007/s11934-018-0841-9] [PMID: 30167899]
[10]
Wang, C.Y.; Bai, X.Y.; Wang, C.H. Traditional Chinese medicine: A treasured natural resource of anticancer drug research and development. Am. J. Chin. Med., 2014, 42(3), 543-559.
[http://dx.doi.org/10.1142/S0192415X14500359] [PMID: 24871650]
[11]
Borchers, A.T.; Hackman, R.M.; Keen, C.L.; Stern, J.S.; Gershwin, M.E. Complementary medicine: A review of immunomodulatory effects of Chinese herbal medicines. Am. J. Clin. Nutr., 1997, 66(6), 1303-1312.
[http://dx.doi.org/10.1093/ajcn/66.6.1303] [PMID: 9394679]
[12]
Cui, X.; Naveed, M.; Baig, M.M.F.A.; Wang, W.; Mikrani, R.; Liu, Z.; Ahmad, B.; Tang, M.; Wazir, J.; Zhou, X.; Han, L. Therapeutic effects of Qianlie Tongli decoction on chronic prostatitis/chronic pelvic pain syndrome induced by peptide T2 in mice. J. Pharm. Pharmacol., 2020, 72(10), 1436-1444.
[http://dx.doi.org/10.1111/jphp.13325] [PMID: 32648958]
[13]
Huang, S.; Meng, N.; Chang, B.; Quan, X.; Yuan, R.; Li, B. Anti-Inflammatory Activity of Epimedium brevicornu Maxim Ethanol Extract. J. Med. Food, 2018, 21(7), 726-733.
[http://dx.doi.org/10.1089/jmf.2017.4088] [PMID: 29620952]
[14]
Mao, Y.F.; Li, Y.Q.; Zong, L.; You, X.M.; Lin, F.Q.; Jiang, L. Methanol extract of Phellodendri cortex alleviates lipopolysaccharide-induced acute airway inflammation in mice. Immunopharmacol. Immunotoxicol., 2010, 32(1), 110-115.
[http://dx.doi.org/10.3109/08923970903193325] [PMID: 19811108]
[15]
Oh, Y.; Kwon, Y.S.; Jung, B.D. Anti-inflammatory Effects of the Natural Compounds Cortex Phellodendri and Humulus japonicus on Pelvic Inflammatory Disease in Mice. Int. J. Med. Sci., 2017, 14(8), 729-734.
[http://dx.doi.org/10.7150/ijms.19616] [PMID: 28824307]
[16]
Balamurugan, M.; Parthasarathi, K.; Cooper, E.L.; Ranganathan, L.S. Earthworm paste (Lampito mauritii, Kinberg) alters inflammatory, oxidative, haematological and serum biochemical indices of inflamed rat. Eur. Rev. Med. Pharmacol. Sci., 2007, 11(2), 77-90.
[PMID: 17552137]
[17]
Liu, Z.K.; Ng, C.F.; Shiu, H.T.; Wong, H.L.; Wong, C.W.; Li, K.K.; Zhang, J.F.; Lam, P.K.; Poon, W.S.; Lau, C.B.S.; Leung, P.C.; Ko, C.H. A traditional Chinese formula composed of Chuanxiong Rhizoma and Gastrodiae Rhizoma (Da Chuanxiong Formula) suppresses inflammatory response in LPS -induced RAW 264.7 cells through inhibition of NF-κB pathway. J. Ethnopharmacol., 2017, 196, 20-28.
[http://dx.doi.org/10.1016/j.jep.2016.12.014] [PMID: 27965052]
[18]
Li, H.; Hung, A.; Yang, A.W.H. A Classic Herbal Formula Danggui Beimu Kushen Wan for Chronic Prostatitis: From Traditional Knowledge to Scientific Exploration. Evid. Based Complement. Alternat. Med., 2018, 2018, 1612948.
[19]
Liu, Y.J.; Song, G.H.; Liu, G.T. Investigation of the effect of traditional Chinese medicine on pain and inflammation in chronic nonbacterial prostatitis in rats. Andrologia, 2016, 48(6), 714-722.
[http://dx.doi.org/10.1111/and.12544] [PMID: 26840892]
[20]
Hill, W.G.; Zeidel, M.L.; Bjorling, D.E.; Vezina, C.M. Void spot assay: Recommendations on the use of a simple micturition assay for mice. Am. J. Physiol. Renal Physiol., 2018, 315(5), F1422-F1429.
[http://dx.doi.org/10.1152/ajprenal.00350.2018] [PMID: 30156116]
[21]
Ihsan, A.U.; Khan, F.U.; Nawaz, W.; Khan, M.Z.; Yang, M.; Zhou, X. Establishment of a rat model of chronic Prostatitis/Chronic Pelvic Pain Syndrome (CP/CPPS) induced by immunization with a novel peptide T2. Biomed. Pharmacother., 2017, 91, 687-692.
[http://dx.doi.org/10.1016/j.biopha.2017.05.004] [PMID: 28499240]
[22]
Zhang, L.; Ihsan, A.U.; Cao, Y.; Cheng, Y.; Zhou, X. Establishment of experimental autoimmune prostatitis model by T 2 peptide in aluminium hydroxide adjuvant. Andrologia, 2018, 50(3), e12922.
[http://dx.doi.org/10.1111/and.12922] [PMID: 29205874]
[23]
Cao, Y.; Cheng, Y.; Ihsan, A.U. A nanoparticle-coupled T2 peptide induces immune tolerance and ameliorates chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) in mice model. Fundam. Clin. Pharmacol., 2019, 33(3), 267-276.
[24]
Cheng, Y.; Cao, Y.; Ihsan, A.U.; Khan, F.U.; Li, X.; Xie, D.; Cui, X.; Wang, W.; Liu, Z.; Li, C.; Ahmad, K.A.; Sembatya, K.R.; Mikrani, R.; Zhou, X. Novel Treatment of Experimental Autoimmune Prostatitis by Nanoparticle-Conjugated Autoantigen Peptide T2. Inflammation, 2019, 42(3), 1071-1081.
[http://dx.doi.org/10.1007/s10753-019-00968-5] [PMID: 30715690]
[25]
Wang, W.; Naveed, M.; Baig, M.M.F.A.; Abbas, M.; Xiaohui, Z. Experimental rodent models of chronic prostatitis and evaluation criteria. Biomed. Pharmacother., 2018, 108, 1894-1901.
[http://dx.doi.org/10.1016/j.biopha.2018.10.010] [PMID: 30453450]
[26]
Murphy, S.F.; Schaeffer, A.J.; Thumbikat, P. Immune mediators of chronic pelvic pain syndrome. Nat. Rev. Urol., 2014, 11(5), 259-269.
[http://dx.doi.org/10.1038/nrurol.2014.63] [PMID: 24686526]
[27]
Aggarwal, B.B.; Van Kuiken, M.E.; Iyer, L.H.; Harikumar, K.B.; Sung, B. Molecular targets of nutraceuticals derived from dietary spices: Potential role in suppression of inflammation and tumorigenesis. Exp. Biol. Med. (Maywood), 2009, 234(8), 825-849.
[http://dx.doi.org/10.3181/0902-MR-78] [PMID: 19491364]
[28]
Kunnumakkara, A.B.; Sailo, B.L.; Banik, K.; Harsha, C.; Prasad, S.; Gupta, S.C.; Bharti, A.C.; Aggarwal, B.B. Chronic diseases, inflammation, and spices: How are they linked? J. Transl. Med., 2018, 16(1), 14.
[http://dx.doi.org/10.1186/s12967-018-1381-2] [PMID: 29370858]
[29]
Wang, W.; Naveed, M. Morphological reseach on expression of inflammatory mediators in murine models of chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) induced by T2 antigen. Andrologia, 2019, 351(11), e13435.
[30]
Nadler, R.B.; Koch, A.E.; Calhoun, E.A.; Campbell, P.L.; Pruden, D.L.; Bennett, C.L.; Yarnold, P.R.; Schaeffer, A.J. IL-1beta and TNF-alpha in prostatic secretions are indicators in the evaluation of men with chronic prostatitis. J. Urol., 2000, 164(1), 214-218.
[http://dx.doi.org/10.1016/S0022-5347(05)67497-6] [PMID: 10840462]
[31]
Coon, J.T.; Ernst, E. Panax ginseng. Drug Saf., 2002, 25(5), 323-344.
[http://dx.doi.org/10.2165/00002018-200225050-00003] [PMID: 12020172]
[32]
Mok, D.K.W.; Chau, F.T. Chemical information of Chinese medicines: A challenge to chemist. Chemom. Intell. Lab. Syst., 2006, 82(1-2), 210-217.
[http://dx.doi.org/10.1016/j.chemolab.2005.05.006]
[33]
Huang, C.F.; Lin, S.S.; Liao, P.H.; Young, S.C.; Yang, C.C. The immunopharmaceutical effects and mechanisms of herb medicine. Cell. Mol. Immunol., 2008, 5(1), 23-31.
[http://dx.doi.org/10.1038/cmi.2008.3] [PMID: 18318991]
[34]
Fox, D.A. The role of T cells in the immunopathogenesis of rheumatoid arthritis. New perspectives. Arthritis Rheum., 1997, 40(4), 598-609.
[http://dx.doi.org/10.1002/art.1780400403] [PMID: 9125240]
[35]
Magistro, G.; Wagenlehner, F.M.E.; Grabe, M.; Weidner, W.; Stief, C.G.; Nickel, J.C. Contemporary Management of Chronic Prostatitis/Chronic Pelvic Pain Syndrome. Eur. Urol., 2016, 69(2), 286-297.
[http://dx.doi.org/10.1016/j.eururo.2015.08.061] [PMID: 26411805]
[36]
Wu, H.; Lien, E.J.; Lien, L.L. Chemical and pharmacological investigations of Epimedium species: A survey. Prog. Drug Res., 2003, 60, 1-57.
[http://dx.doi.org/10.1007/978-3-0348-8012-1_1] [PMID: 12790338]
[37]
Ma, H.; He, X.; Yang, Y.; Li, M.; Hao, D.; Jia, Z. The genus Epimedium: An ethnopharmacological and phytochemical review. J. Ethnopharmacol., 2011, 134(3), 519-541.
[http://dx.doi.org/10.1016/j.jep.2011.01.001] [PMID: 21215308]
[38]
Ding, J.; Tang, Y.; Tang, Z.; Zu, X.; Qi, L.; Zhang, X.; Wang, G. Icariin improves the sexual function of male mice through the PI3K/AKT/eNOS/NO signalling pathway. Andrologia, 2018, 50(1), e12802.
[http://dx.doi.org/10.1111/and.12802] [PMID: 28294371]
[39]
Shen, R.; Wang, J.H. The effect of icariin on immunity and its potential application. Am. J. Clin. Exp. Immunol., 2018, 7(3), 50-56.
[PMID: 30038846]
[40]
Cunha, T.M.; Verri, W.A., Jr; Valério, D.A.; Guerrero, A.T.; Nogueira, L.G.; Vieira, S.M.; Souza, D.G.; Teixeira, M.M.; Poole, S.; Ferreira, S.H.; Cunha, F.Q. Role of cytokines in mediating mechanical hypernociception in a model of delayed‐type hypersensitivity in mice. Eur. J. Pain, 2008, 12(8), 1059-1068.
[http://dx.doi.org/10.1016/j.ejpain.2008.02.003] [PMID: 18372199]
[41]
He, X.H.; Zang, Y.; Chen, X.; Pang, R.P.; Xu, J.T.; Zhou, X.; Wei, X.H.; Li, Y.Y.; Xin, W.J.; Qin, Z.H.; Liu, X.G. TNF-α contributes to up-regulation of Nav1.3 and Nav1.8 in DRG neurons following motor fiber injury. Pain, 2010, 151(2), 266-279.
[http://dx.doi.org/10.1016/j.pain.2010.06.005] [PMID: 20638792]
[42]
Ren, L.; Xue, X.; Zhang, F.; Xu, Q.; Liang, X. High performance liquid chromatography‐mass spectrometry analysis of protoberberine alkaloids in medicine herbs. J. Sep. Sci., 2007, 30(6), 833-842.
[http://dx.doi.org/10.1002/jssc.200600246] [PMID: 17536728]
[43]
Jung, H.W.; Kim, K.H.; Park, Y.K. Inhibitory effect of the extract of Phellodendron amurense ruprecht root on collagen-induced arthritis in mice. Chin. J. Integr. Med., 2017, 23(10), 755-762.
[http://dx.doi.org/10.1007/s11655-016-2094-5] [PMID: 27460490]
[44]
Scott, D.L.; Wolfe, F.; Huizinga, T.W.J. Rheumatoid arthritis. Lancet, 2010, 376(9746), 1094-1108.
[http://dx.doi.org/10.1016/S0140-6736(10)60826-4] [PMID: 20870100]
[45]
Hui, Sun A Metabolomic Study on the Intervention Effect of Phellodenron Amurense Rupr. Alkaloid Protects Against Chronic Nonbacterial Prostatitis In Rats. Modern. Trad. Chin. Med. Mater Medica., 2016, 18(10), 1709-1719.
[46]
Li, C.; Chen, M.; Li, X.; Yang, M.; Wang, Y.; Yang, X. Purification and function of two analgesic and anti-inflammatory peptides from coelomic fluid of the earthworm, Eisenia foetida. Peptides, 2017, 89, 71-81.
[http://dx.doi.org/10.1016/j.peptides.2017.01.016] [PMID: 28161427]

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