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Current Organic Chemistry

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ISSN (Print): 1385-2728
ISSN (Online): 1875-5348

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Research Article

NMR Structure Elucidation and Molecular Modeling of Lipoxygenase and Cholinesterase Inhibiting Steroids from Hypericum oblongifolium

Author(s): Anam Sajid, Muhammad Afzal, Arfaa Sajid*, Qaisar Manzoor, Ejaz ahmed, Ahsan Sharif and Samia Younas

Volume 26, Issue 19, 2022

Published on: 23 December, 2022

Page: [1798 - 1806] Pages: 9

DOI: 10.2174/1385272827666221216111557

Price: $65

Abstract

Hypericum oblongifolium is a potent source of bioactive constituents. A series of pharmacological properties, ranging from wound healing and antiseptic to antiviral, antiinflammatory, anticancer, and apoptosis-inducing activities have been associated with this plant. The current research project was designed to investigate the new secondary metabolites in H. oblongifolium having excellent pharmaceutical potential. In the present investigation two new cholestane-type steroids, hyperinoate A (1) and hyperinoate B (2) were isolated from a chloroform soluble fraction of the whole plant of H. oblongifolium. Structures of isolated new compounds were elucidated based on spectroscopic data including 1D (1HNMR, 13CNMR) and 2D (HMBC, COSY, NOESY) NMR and mass spectrometry (EIMS, HREIMS) data. After structure elucidation, new compounds were named 6α-hydroxy-14α-methyl Cholestan-3-ylacetate and 6α,25-dihydroxy-14α-methyl Cholestane-3-yl-acetate. Both steroids showed promising inhibitory activity against lipoxygenase (LOX) and acetylcholinesterase (AChE) enzymes. Especially hyperinoate A (1) inhibited the lipoxygenase (LOX) enzyme with IC50 41.7± 0.15 μM whereas Baicalein (positive control) had IC50 2.20 ± 0.04 μM. Similarly, Hyperinoate B (2) (56.3 ± 0.33 μM) showed moderate inhibition compared to Galantamine (positive control) 5.38 ± 0.54 μM. These results were validated with in-silico molecular docking investigations. The binding affinity of hyperinoate A (1) (-9.2 Kcal/mol) against LOX-5(PDB ID 3V99) showed moderate inhibition as compared to baicalein (positive control) (-7.7 Kcal/mol).The binding affinities of hyperinoate B (2) (-10.5 Kcal/mol) were close to galantamine (-10.6 Kcal/mol). All in-vitro and in-silico results revealed that both newly isolated compounds showed moderate inhibition against lipoxygenase (LOX) and acetylcholinesterase (AChE) enzymes.

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