Protein photodegradation in the fluorescence unfolding kinetics study of bromelain

Alfonso      Arroyo-Reyna; Salvador   R.   Tello-Solis

doi:10.2174/092986650702221206114551

Abstract

We have detected protein photodegradation in the fluorescence unfolding kinetics experiment of stem bromelain, a cysteine proteinase from the papain family. Thermally induced unfolding has been followed by near and far circular dichroism and tryptophan fluorescence emission. It was found that the fluorescence kinetics involves two phases. This biphasic nature results from ultraviolet induced photolysis oftryptophan.

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Protein & Peptide Letters

Title:Protein photodegradation in the fluorescence unfolding kinetics study of bromelain

Volume: 7 Issue: 2

Author(s): Alfonso Arroyo-Reyna*Salvador R. Tello-Solis

Affiliation:

Area de Biofisicoquimica, Departamento de Quimica, Universidad Aut6noma Metropolitana-Iztapalapa, Apartado Postal 55-534, Iztapalapa 09340, D.F., Mexico

Abstract: We have detected protein photodegradation in the fluorescence unfolding kinetics experiment of stem bromelain, a cysteine proteinase from the papain family. Thermally induced unfolding has been followed by near and far circular dichroism and tryptophan fluorescence emission. It was found that the fluorescence kinetics involves two phases. This biphasic nature results from ultraviolet induced photolysis oftryptophan.

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Arroyo-Reyna Alfonso*, Tello-Solis R. Salvador, Protein photodegradation in the fluorescence unfolding kinetics study of bromelain, Protein & Peptide Letters 2000; 7 (2) . https://dx.doi.org/10.2174/092986650702221206114551

DOI https://dx.doi.org/10.2174/092986650702221206114551	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305