The crystal structure of met-rantes: comparison with native rantes and aop-rantes

David   M.   Hoover; Jeffrey      Shaw; Zygmunt      Gryczynski; Amanda   E. I.   Proudfoot; Tim      Wells; Jacek      Lubkowski

doi:10.2174/092986650702221206112548

Abstract

Met-RANTES is a modified version of the chemokine RANTES, which has an additional amino acid at the amino terminus. To compare Met-RANTES with the native protein as well as with another antagonist, AOP RANTES, we have solved the structure of Met-RANTES at 1.6 A by X-ray crystallography and investigated the dimerization of all three proteins by fluorescence anisotropy. The dimerization of AOP-RANTES shows an altered aggregation profile. The delineation of slight differences in binding sulfate anions by Met- and AOP RANTES might be linked to specific yet different interactions between these proteins and glycosaminoglycans.

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Protein & Peptide Letters

Title:The crystal structure of met-rantes: comparison with native rantes and aop-rantes

Volume: 7 Issue: 2

Author(s): David M. Hoover, Jeffrey Shaw, Zygmunt Gryczynski, Amanda E. I. Proudfoot, Tim Wells and Jacek Lubkowski

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Abstract: Met-RANTES is a modified version of the chemokine RANTES, which has an additional amino acid at the amino terminus. To compare Met-RANTES with the native protein as well as with another antagonist, AOP RANTES, we have solved the structure of Met-RANTES at 1.6 A by X-ray crystallography and investigated the dimerization of all three proteins by fluorescence anisotropy. The dimerization of AOP-RANTES shows an altered aggregation profile. The delineation of slight differences in binding sulfate anions by Met- and AOP RANTES might be linked to specific yet different interactions between these proteins and glycosaminoglycans.

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Hoover M. David, Shaw Jeffrey, Gryczynski Zygmunt, Proudfoot E. I. Amanda, Wells Tim and Lubkowski Jacek, The crystal structure of met-rantes: comparison with native rantes and aop-rantes, Protein & Peptide Letters 2000; 7 (2) . https://dx.doi.org/10.2174/092986650702221206112548

DOI https://dx.doi.org/10.2174/092986650702221206112548	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305