A comparative study of the function of phospholipases a2 fromagkistrodon acutus

Title:A comparative study of the function of phospholipases a2 fromagkistrodon acutus

Volume: 7 Issue: 2

Author(s): Xiaolong Liu, Ying Xiong, Xiangfu Wu and Yuancong Zhou*

Affiliation:

• Shanghai Institute of Biochemistry The Chinese Academy of Sciences 320 Yue-yang Road, Shanghai 200031 P.R. China

Abstract: cDNAs encoding acidic phospholipases A2I and acid A2II, basic phospholipase A2 and Lys49- phospholipase A2 from Agkistrodon acutus were inserted into bacterial expression vectors and effectively expressed in E.coli RRl. Except for Lys49-phospholipase A2, the other phospholipase A2s have high enzymatic activities. Acidic phospholipas_eA2I has an inhibiting effect on platelet aggregation. All phospholipase A2s except acidic phospholipase A2II have hemolytic activities. The roles of various amino acid residues in the enzymatic, hemolytic and platelet aggregation inhibiting activities of phospholipase A2 are discussed.

Cite this article as:

Liu Xiaolong, Xiong Ying, Wu Xiangfu and Zhou Yuancong*, A comparative study of the function of phospholipases a2 fromagkistrodon acutus, Protein & Peptide Letters 2000; 7 (2) . https://dx.doi.org/10.2174/092986650702221206113125