Occurrence of an essential amino acid residues in the lytic effect of escherichia coli a -haemolysin

Title:Occurrence of an essential amino acid residues in the lytic effect of escherichia coli a -haemolysin

Volume: 7 Issue: 2

Author(s): Laura Bakas*

Affiliation:

• Instituto de Investigaciones Bioquimicas de La Plata (INIBIOLP), Facultad de Ciencias Medicas-CONICET 60 y 120 (1900) ,La Plata-Argentina
• Dto de Cs. Biol6gicas, Facultad de Cs. Exactas UNLP, 47 y 115 (1900) La Plata, Argentina

Abstract: α-Hemolysin (HlyA), an extracellular protein toxin produced by some pathogenic strains of Escherichia coli , is known to disrupt eukaryotic cell membranes. In an attempt to correlate the structure of this toxin with its function, we studied the role of the histidine residues on the toxin activity by their chemical modification with the reagent diethyl pyrocarbonate (DEPC). The results of this study suggest the presence of an essential histidine residue at the Ca²⁺-binding motif and it.s role in the lytic action of E.coli HlyA.

Cite this article as:

Bakas Laura*, Occurrence of an essential amino acid residues in the lytic effect of escherichia coli a -haemolysin, Protein & Peptide Letters 2000; 7 (2) . https://dx.doi.org/10.2174/092986650702221206113546