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ISSN (Online): 1875-6646

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Research Article

Cytotoxic Effects of Homeopathic Preparations on Human Tumor Cells In Vitro

Author(s): Olga Botkina and Evgenii Plotnikov*

Volume 19, Issue 3, 2023

Published on: 09 September, 2022

Article ID: e090522204471 Pages: 9

DOI: 10.2174/1573407218666220509184504

Price: $65

Abstract

The aim of this work was to conduct an in vitro assessment of the possible cytotoxic effects of homeopathic drugs and consider possible mechanisms.

Background: Homeopathy is still widely used as a complementary (alternative) medicine in different countries throughout the world. However, the method raises a lot of scientific debate about its effectiveness and mechanisms of action. This is especially true concerning the use of homeopathy in cancer treatment.

Objective: This work aimed to comparatively assess the cytotoxic effect of homeopathic remedies on tumor cell cultures.

Materials and Methods: We studied different dilutions of homeopathic medicines: Hydrastis, Conium and Carcinosinum. Cell lines of prostate cancer PC-3, breast cancer MDA-MB-231, and lymphoblastic leukemia Jurkat were used as model tumor objects. Standard colorimetric MTT test and cytometric analysis of cells were used here to assess the viability and parameters of apoptosis of cells.

Results: Comparative assessment of the cytotoxic and apoptosis-inducing effects did not reveal significant differences in the viability of tumor cells in the groups exposed to homeopathic preparations among themselves and in comparison with the control group. The effects of the action of homeopathic remedies are shown only in the form of trends. Some possible mechanisms of the obtained results are considered.

Conclusion: The results obtained in vitro indicated the absence of a direct cytotoxic effect in the studied homeopathic preparations. Despite the objective data, this does not allow us to make a final conclusion about the absence of antitumor activity of these drugs in vivo, since the possible or potential effects depend on the parameters of a living organism that are difficult to control and are often based only on hypothetical mechanisms. Some aspects of the action of homeopathy probably cannot be reproduced in vitro.

Keywords: Hydrastis, conium, carcinosinum, PC-3, MDA-MB-231, jurkat, homeopathy, apoptosis.

Graphical Abstract

[1]
Fisher, P. What is homeopathy? An introduction. Front. Biosci. (Elite Ed.), 2012, 4(5), 1669-1682.
[http://dx.doi.org/10.2741/e489] [PMID: 22201984]
[2]
Walach, H.; Jonas, W.B.; Ives, J.; van Wijk, R.; Weingärtner, O. Research on homeopathy: state of the art. J. Altern. Complement. Med., 2005, 11(5), 813-829.
[http://dx.doi.org/10.1089/acm.2005.11.813] [PMID: 16296915]
[3]
World Health Organization. The top 10 causes of death., 2022. Available from: https://www.who.int/news-room/fact-sheets/detail/the-top-10-causes-of-death (Accessed on February 13, 2022).
[4]
Jiang, M.; Zhang, C.; Cao, H.; Chan, K.; Lu, A. The role of Chinese medicine in the treatment of chronic diseases in China. Planta Med., 2011, 77(9), 873-881.
[http://dx.doi.org/10.1055/s-0030-1270983] [PMID: 21472645]
[5]
Choi, B.; Han, D.; Na, S.; Lim, B. Factors related to the parallel use of complementary and alternative medicine with conventional medicine among patients with chronic conditions in South Korea. Integr. Med. Res., 2017, 6(2), 223-229.
[http://dx.doi.org/10.1016/j.imr.2017.04.002] [PMID: 28664145]
[6]
Wazaify, M.; Alawwa, I.; Yasein, N.; Al-Saleh, A.; Afifi, F.U. Complementary and alternative medicine (CAM) use among Jordanian patients with chronic diseases. Complement. Ther. Clin. Pract., 2013, 19(3), 153-157.
[http://dx.doi.org/10.1016/j.ctcp.2013.03.001] [PMID: 23890462]
[7]
Silva, M.L.A.E.; Lucarini, R.; Dos Santos, F.F.; Martins, C.H.G.; Pauletti, P.M.; Januario, A.H.; Santos, M.F.C.; Cunha, W.R. Hypoglycemic effect of rosmarinic acid-rich infusion (RosCE) from Origanum vulgare in alloxan-induced diabetic rats. Nat. Prod. Res., 2021, 1-7.
[http://dx.doi.org/10.1080/14786419.2021.1990282] [PMID: 34647501]
[8]
Banik, K.; Khatoon, E.; Harsha, C.; Rana, V.; Parama, D.; Thakur, K.K.; Bishayee, A.; Kunnumakkara, A.B. Wogonin and its analogs for the prevention and treatment of cancer: A systematic review. Phytother. Res., 2022, 36(5), 1854-1883.
[http://dx.doi.org/10.1002/ptr.7386] [PMID: 35102626]
[9]
Bharat, S.; Pooran, S.; Ram, S. Phytochemical profiling, anti-inflammatory, antioxidant and antimicrobial effects of seven Indian Morinda species. Curr. Bioact. Compd., 2022, 18(2)e251121196689.
[http://dx.doi.org/10.2174/1573407217666210921113535]
[10]
Pomposelli, R.; Piasere, V.; Andreoni, C.; Costini, G.; Tonini, E.; Spalluzzi, A.; Rossi, D.; Quarenghi, C.; Zanolin, M.E.; Bellavite, P. Observational study of homeopathic and conventional therapies in patients with diabetic polyneuropathy. Homeopathy, 2009, 98(1), 17-25.
[http://dx.doi.org/10.1016/j.homp.2008.11.006] [PMID: 19135955]
[11]
Joubert, K.S.; George, B.P.; Razlog, R.; Abrahamse, H. A Review of preclinical research on the effects of photodynamic therapy and homeopathic medicine on cancer cells. Altern. Ther. Health Med., 2021, 27(6), 40-50.
[PMID: 32619203]
[12]
Tenzera, L.; Djindjic, B.; Mihajlovic-Elez, O.; Pulparampil, B.J.; Mahesh, S.; Vitoulkas, G. Improvements in long standing cardiac pathologies by individualized homeopathic remedies: A case series. SAGE Open Med. Case Rep, 2018. 6, 2050313X1879281.
[http://dx.doi.org/ 10.1177/2050313X18792813]
[13]
Frass, M.; Linkesch, M.; Banyai, S.; Resch, G.; Dielacher, C.; Löbl, T.; Endler, C.; Haidvogl, M.; Muchitsch, I.; Schuster, E. Adjunctive homeopathic treatment in patients with severe sepsis: a randomized, double-blind, placebo-controlled trial in an intensive care unit. Homeopathy, 2005, 94(2), 75-80.
[http://dx.doi.org/10.1016/j.homp.2005.01.002] [PMID: 15892486]
[14]
Frass, M.; Lechleitner, P.; Gründling, C.; Pirker, C.; Grasmuk-Siegl, E.; Domayer, J.; Hochmair, M.; Gaertner, K.; Duscheck, C.; Muchitsch, I.; Marosi, C.; Schumacher, M.; Zöchbauer-Müller, S.; Manchanda, R.K.; Schrott, A.; Burghuber, O. Homeopathic treatment as an add-on therapy may improve quality of life and prolong survival in patients with non-small cell lung cancer: a prospective, randomized, placebo-controlled, double-blind, three-arm, multicenter study. Oncologist, 2020, 25(12), e1930-e1955.
[http://dx.doi.org/10.1002/onco.13548] [PMID: 33010094]
[15]
Shang, A.; Huwiler-Müntener, K.; Nartey, L.; Jüni, P.; Dörig, S.; Sterne, J.A.; Pewsner, D.; Egger, M. Are the clinical effects of homoeopathy placebo effects? Comparative study of placebo-controlled trials of homoeopathy and allopathy. Lancet, 2005, 366(9487), 726-732.
[http://dx.doi.org/10.1016/S0140-6736(05)67177-2] [PMID: 16125589]
[16]
Ernst, E. Classical homeopathy versus conventional treatments: A systematic review. Perfusion, 1999, 12, 13-15.
[17]
Ernst, E. A systematic review of systematic reviews of homeopathy. Br. J. Clin. Pharmacol., 2002, 54(6), 577-582.
[http://dx.doi.org/10.1046/j.1365-2125.2002.01699.x] [PMID: 12492603]
[18]
Chikramane, P.S.; Suresh, A.K.; Bellare, J.R.; Kane, S.G. Extreme homeopathic dilutions retain starting materials: A nanoparticulate perspective. Homeopathy, 2010, 99(4), 231-242.
[http://dx.doi.org/10.1016/j.homp.2010.05.006] [PMID: 20970092]
[19]
Elia, V.; Baiano, S.; Duro, I.; Napoli, E.; Niccoli, M.; Nonatelli, L. Permanent physico-chemical properties of extremely diluted aqueous solutions of homeopathic medicines. Homeopathy, 2004, 93(3), 144-150.
[http://dx.doi.org/10.1016/j.homp.2004.04.004] [PMID: 15287434]
[20]
Lo, S.Y. Anomalous state of ice. Mod. Phys. Lett. B, 1996, 10(19), 909-919.
[http://dx.doi.org/10.1142/S0217984996001036]
[21]
Lo, S.Y.; Chong, L.W.; Tianzhang, L.; Hua, L.H.; Geng, X. Physical properties of water with IE structures. Mod. Phys. Lett. B, 1996, 10(19), 921-930.
[http://dx.doi.org/10.1142/S0217984996001048]
[22]
Rey, L. Thermoluminescence of ultra high dilutions of lithium chloride and sodium chloride. Physica A, 2003, 323, 67-74.
[http://dx.doi.org/10.1016/S0378-4371(03)00047-5]
[23]
Samal, S.; Geckeler, K.E. Unexpected solute aggregation in water on dilution. Chem. Commun. (Camb.), 2001, 7(21), 2224-2225.
[http://dx.doi.org/10.1039/b105399j] [PMID: 12240122]
[24]
Michael, J.; Singh, S.; Sadhukhan, S.; Nath, A.; Kundu, N.; Magotra, N.; Dutta, S.; Parewa, M.; Koley, M.; Saha, S. Efficacy of individualized homeopathic treatment of insomnia: Double-blind, randomized, placebo-controlled clinical trial. Complement. Ther. Med., 2019, 43, 53-59.
[http://dx.doi.org/10.1016/j.ctim.2019.01.007] [PMID: 30935555]
[25]
Henrique da Silva, G.; Barros, P.P.; Silva Gonçalves, G.M.; Landi, M.A. Hepatoprotective effect of Lycopodium clavatum 30CH on experimental model of paracetamol-induced liver damage in rats. Homeopathy, 2015, 104(1), 29-35.
[http://dx.doi.org/10.1016/j.homp.2014.05.005] [PMID: 25576269]
[26]
Hofbauer, R.; Pasching, E.; Moser, D.; Frass, M. Heparin-binding epidermal growth factor expression in KATO-III cells after Helicobacter pylori stimulation under the influence of Strychnos Nux vomica and Calendula officinalis. Homeopathy, 2010, 99(3), 177-182.
[http://dx.doi.org/10.1016/j.homp.2010.05.002] [PMID: 20674841]
[27]
Arruda-Silva, F.; Bellavite, P.; Marzotto, M. Low-dose Drosera rotundifolia induces gene expression changes in 16HBE human bronchial epithelial cells. Sci. Rep., 2021, 11(1), 2356.
[http://dx.doi.org/10.1038/s41598-021-81843-y] [PMID: 33504888]
[28]
Arora, S.; Aggarwal, A.; Singla, P.; Jyoti, S.; Tandon, S. Anti-proliferative effects of homeopathic medicines on human kidney, colon and breast cancer cells. Homeopathy, 2013, 102(4), 274-282.
[http://dx.doi.org/10.1016/j.homp.2013.06.001] [PMID: 24050774]
[29]
Valle, A.; Aguiar, L.R.; Brunel, H.D.; Malard, P.F.; Andrade, R.V. Analysis of the performance of the ultra-diluted Viscum album in cultivation of mesenchymal stem cells and mammary adenocarcinoma cells PMC-42 and MCF-7. Int. J. Hum. Sci., 2020, 4(2), 279-283.
[30]
Khan, S.; Nayak, D.; Khurana, A.; Manchanda, R.K.; Tandon, C.; Tandon, S. In vitro assessment of homeopathic potencies of hydrastis canadensis on hormone-dependent and independent breast cancer. Homeopathy, 2020, 109(4), 198-206.
[http://dx.doi.org/10.1055/s-0040-1709668] [PMID: 32610349]
[31]
Burnett, James Epps & Co.: 170 Piccadilly and 48 Threadneedle Street: London, 1888.
[32]
Clarke, J.H. The Cure of Tumours by Medicines whith especial reference to the cancer nosodes; James Epps & Co.: 48 Threadneedle Street and 60 Jermyn Street: London , 1908.
[33]
Frenkel, M.; Mishra, B.M.; Sen, S.; Yang, P.; Pawlus, A.; Vence, L.; Leblanc, A.; Cohen, L.; Banerji, P.; Banerji, P. Cytotoxic effects of ultra-diluted remedies on breast cancer cells. Int. J. Oncol., 2010, 36(2), 395-403.
[PMID: 20043074]
[34]
Yadav, R.; Jee, B.; Rao, K.S. How homeopathic medicine works in cancer treatment: deep insight from clinical to experimental studies. J. Exp. Ther. Oncol., 2019, 13(1), 71-76.
[PMID: 30658031]
[35]
Relton, C.; Cooper, K.; Viksveen, P.; Fibert, P.; Thomas, K. Prevalence of homeopathy use by the general population worldwide: a systematic review. Homeopathy, 2017, 106(2), 69-78.
[http://dx.doi.org/10.1016/j.homp.2017.03.002] [PMID: 28552176]
[36]
Ernst, E. Homeopathy for cancer? Curr. Oncol., 2007, 14(4), 128-130.
[http://dx.doi.org/10.3390/curroncol14040004] [PMID: 17710204]
[37]
Milazzo, S.; Russell, N.; Ernst, E. Efficacy of homeopathic therapy in cancer treatment. Eur. J. Cancer, 2006, 42(3), 282-289.
[http://dx.doi.org/10.1016/j.ejca.2005.09.025] [PMID: 16376071]
[38]
MacLaughlin, B.W.; Gutsmuths, B.; Pretner, E.; Jonas, W.B.; Ives, J.; Kulawardane, D.V.; Amri, H. Effects of homeopathic preparations on human prostate cancer growth in cellular and animal models. Integr. Cancer Ther., 2006, 5(4), 362-372.
[http://dx.doi.org/10.1177/1534735406295350] [PMID: 17101766]
[39]
Burlakova, E.B.; Kondradov, A.A.; Mal’tseva, E.L. [Superweak effects of chemical compounds and physical factors on biological systems]. Biofizika, 2004, 49(3), 551-564.
[PMID: 15327217]
[40]
Vladimirsky, E.B.; Mil’man, V.D. Mechanisms of signal transduction in cells. facts and hypotheses. Klin. Onkogematol, 2015, 8(3), 248-254.
[41]
Montagnier, L.; Aïssa, J.; Ferris, S.; Montagnier, J.L.; Lavallée, C. Electromagnetic signals are produced by aqueous nanostructures derived from bacterial DNA sequences. Interdiscip. Sci., 2009, 1(2), 81-90.
[http://dx.doi.org/10.1007/s12539-009-0036-7] [PMID: 20640822]
[42]
Sunila, E.S.; Kuttan, R.; Preethi, K.C.; Kuttan, G. Dynamized preparations in cell culture. Evid. Based Complement. Alternat. Med., 2009, 6(2), 257-263.
[http://dx.doi.org/10.1093/ecam/nem082] [PMID: 18955237]
[43]
Preethi, K.; Ellanghiyil, S.; Kuttan, G.; Kuttan, R. Induction of apoptosis of tumor cells by some potentiated homeopathic drugs: implications on mechanism of action. Integr. Cancer Ther., 2012, 11(2), 172-182.
[http://dx.doi.org/10.1177/1534735411400310] [PMID: 21771822]
[44]
Angell, M.; Kassirer, J.P. Alternative medicine--the risks of untested and unregulated remedies. N. Engl. J. Med., 1998, 339(12), 839-841.
[http://dx.doi.org/10.1056/NEJM199809173391210] [PMID: 9738094]
[45]
Burlakova, E.B. Features of the action of ultra-low doses of biologically active substances and physical factors of low intensity. Russian Chem J, 1999, 5, 3-11.
[46]
Cukaci, C.; Freissmuth, M.; Mann, C.; Marti, J.; Sperl, V. Against all odds-the persistent popularity of homeopathy. Wien. Klin. Wochenschr., 2020, 132(9-10), 232-242.
[http://dx.doi.org/10.1007/s00508-020-01624-x] [PMID: 32152694]
[47]
Stub, T.; Kristoffersen, A.E.; Overvåg, G.; Jong, M.C.; Musial, F.; Liu, J. Adverse effects in homeopathy. A systematic review and meta-analysis of observational studies. Explore (NY), 2022, 18(1), 114-128.
[http://dx.doi.org/10.1016/j.explore.2020.11.008] [PMID: 33303386]
[48]
Javed, B.; Ikram, M.; Farooq, F.; Sultana, T.; Mashwani, Z.U.; Raja, N.I. Biogenesis of silver nanoparticles to treat cancer, diabetes, and microbial infections: a mechanistic overview. Appl. Microbiol. Biotechnol., 2021, 105(6), 2261-2275.
[http://dx.doi.org/10.1007/s00253-021-11171-8] [PMID: 33591386]
[49]
Plotnikov, E.; Plotnikov, V. Investigation of antiblastic and antibacterial properties of binuclear platinum (II)-interferon conjugate. Curr. Bioact. Compd., 2022, 18e120122200174.

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