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Current Nanoscience

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ISSN (Print): 1573-4137
ISSN (Online): 1875-6786

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General Research Article

Combined Effect of Zinc Oxide Nanoparticles and Low Electric Field in Growth Suppression of Some Free-living Pathogens

Author(s): Mamdouh M. Shawki*, Marwa M. Eltarahony and Maisa E. Moustafa

Volume 18, Issue 4, 2022

Page: [535 - 544] Pages: 10

DOI: 10.2174/1573413717666211026151538

Price: $65

Abstract

Background: The re-emergence of infectious diseases and the increasing rate of the appearance of many antibiotic-resistant strains are major public health concerns. Zinc oxide nanoparticles (ZnO-NPs) have a great antibacterial effect. Few reports stated the antibacterial effect of low electric field (LEF).

Objective: The paper aimed to study the antibacterial effect of LEF at low frequency and investigate the antibacterial effectiveness of using LEF in synergy with ZnO-NPs.

Methods: Pseudomonas aeruginosa and Staphylococcus aureus were examined as models for Gramnegative and Gram-positive bacteria, respectively. The bacterial suspension was exposed to different concentrations of Zn-NPs ranging from 100-1600 μg/ml or 2 V/cm, 500 Hz AC electric field for 5 min. ZnO-NPs were prepared and characterized by UV-Vis spectroscopy, XRD, FTIR, TEM, and SEM. The combined effect of LEF exposure with each ZnO-NPs concentration was assessed.

Results: 1600 μg/ml ZnO-NPs cause 41.93% and 48.15% death, LEF produces 20.88% and 28.03% death, and the synergetic effect causes 50.41% and 70.27% death for P. aeruginosa and S. aureus, respectively. The death percentages were correlated with DNA concentration and deformation, reactive oxygen species concentration, and ultrastructure changes.

Conclusions: LEF has antibacterial properties and can be used in combination with ZnO-NPs to increase its lethal effect.

Keywords: Low electric field, ZnO nanoparticles, antimicrobial activity, DNA concentration and deformation, reactive oxygen species concentration, ultrastructure changes.

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