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Research Article

Del-1诱导银屑病内皮细胞αvβ3和α5β1表达

卷 22, 期 5, 2022

发表于: 20 August, 2021

页: [442 - 448] 页: 7

弟呕挨: 10.2174/1566524021666210729112700

价格: $65

摘要

目的：银屑病是一种高度依赖血管生成的慢性炎症性皮肤病。我们先前的研究结果表明，银屑病患者真皮间充质干细胞（dMSCs）中Del-1的mRNA和蛋白表达上调。我们的目的是进一步研究来自dMSCs的Del-1在银屑病发病机制中的作用，并证实Del-1在银屑病发病机制中的作用。 方法：我们进行了一项免疫组织化学实验，以进一步研究Del-1在银屑病患者皮损中的表达。此外，通过Del-1慢病毒载体将Del-1过表达的dMSCs与ECs共培养，通过蛋白质印迹法检测ECs整合素（αvβ3、αvβ5和α5β1）的蛋白表达。 结果：本研究表明，银屑病患者皮损中的Del-1显著增加（p<0.05，9.96 vs.2.18），dMSCs中的Del-1成功诱导整合素α5β1和αvβ3的上调（均p<0.05）。 结论：本研究阐述了dMSCs中的Del-1通过诱导血管生成参与银屑病的发病机制。Del-1、αvβ3和α5β1可能是抑制银屑病血管生成的潜在新靶点。

关键词: 银屑病，Del-1，真皮间充质干细胞（dMSCs），整合素，血管生成，蛋白质印迹法。

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