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Current Molecular Medicine

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ISSN (Print): 1566-5240
ISSN (Online): 1875-5666

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Research Article

Del-1 in Psoriasis Induced the Expression of αvβ3 and α5β1 in Endothelial Cells

Author(s): Xuping Niu, Qixin Han, Xiaofang Li, Juan Li, Yanmin Liu, Yan Li and Yan Wu*

Volume 22, Issue 5, 2022

Published on: 20 August, 2021

Page: [442 - 448] Pages: 7

DOI: 10.2174/1566524021666210729112700

Price: $65

Abstract

Objective: Psoriasis is a chronic inflammatory skin disease highly depending on angiogenesis. Our prior results showed that the mRNA and protein of Del-1 in dermal mesenchymal stem cells (dMSCs) was up-regulated from psoriasis. Our aim was further to investigate the role of Del-1 from dMSCs in the pathogenesis of psoriasis and confirm the effect of Del-1 on the pathogenesis of psoriasis.

Methods: We conducted an immunohistochemistry experiment to further investigate the expression of Del-1in psoriatic lesions. In addition, dMSCs with over-expressed Del-1 via the lentiviral vector of Del-1 were co-cultured with ECs, and the protein expression of integrins (αvβ3, αvβ5 ,and α5β1) of ECs were detected by western blotting.

Results: This research showed that Del-1 was significantly increased in lesions of patients with psoriasis (p< .05, 9.96 vs. 2.18), and Del-1 from dMSCs successfully induced up-regulation of integrins α5β1 and αvβ3 (all p < .05).

Conclusion: This study demonstrated that Del-1 from dMSCs was involved in the pathogenesis of psoriasis through induced angiogenesis. And Del-1, αvβ3 and α5β1 may be potential new targets for inhibiting angiogenesis in psoriasis.

Keywords: Psoriasis, Del-1, dermal mesenchymal stem cells (dMSCs), integrin, angiogénesis, western blotting.

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