Abstract
Transplantation of mesenchymal stem/stromal cells (MSCs) is an emerging treatment for various hard-to-treat diseases. In recent decades, cell sheet technology (CST) has been exploited as a novel promising scaffold-free approach for cell culturing and delivering in tissue regeneration. Results have demonstrated that MSCs facilitate cell viability and function in the CST co-culture system to improve transplantation efficiency. There are extensive interests in developing strategies to enhance the formation of cell sheets with MSC for downstream applications. Novel fabrication techniques have been emerging in recent years, including temperature-responsive system, electroactive responsive system, oxide surface assistant electrochemical system, light responsive system, PH responsive system, magnetite nanoparticles and magnetic force system. This chapter summarizes recent studies in cell sheet engineering and its combination with MSC for tissue regeneration.
Keywords: Cell sheet technology, Co-culture, Mesenchymal stem cell, Micropatterning, Tissue engineering, Vascularization.
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