Recent Advances in Biotechnology

Volume: 1

PHBHV Biosynthesis by Haloferax mediterranei: from Genetics, Metabolism, and Engineering to Economical Production

Author(s): Hua Xiang

Pp: 348-379 (32)

DOI: 10.2174/9781681083254116010009

* (Excluding Mailing and Handling)

Abstract

The halophilic archaeon Haloferax mediterranei, has shown promise for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBHV), a desirable bioplastic, from many low-cost carbon sources. To obtain a full understanding of PHBHV biosynthesis and to provide novel strategies for polyhydroxyalkanaote (PHA) production by haloarchaea, the complete genome of H. mediterranei has been determined, and a genome-wide investigation of the genetics and metabolism of this haloarchaeon involved in PHBHV biosynthesis was performed in the past few years. This exploration covers the identification of key genes for PHBHV biosynthesis, the specific precursor supplying pathways, the PHA granule modulation, and PHBHV mobilization, as well as its linkage to haloarchaeal-specific acetyl-CoA and propionyl- CoA assimilation. These advances have not only revealed many haloarchaeal-specific enzymes and pathways for PHBHV biosynthesis, such as the archaeal-type PHA synthases, the haloarchaeal-type β-ketothiolases with two distinct subunits, and the novel 3-hydroxypropionate pathway coupling CO2 fixation into PHBHV, but have also provided strategies for bioengineering haloarchaea for economical production of PHBHV or tailor-made higher-value PHA. This chapter will summarize these current progresses of PHBHV biosynthesis by H. mediterranei, thus providing a perspective for economical production of industry-scale or higher-value PHAs by haloarchaea in the near future.


Keywords: Acetoacetyl-CoA reductase, Acetyl-CoA, β-ketothiolase, β-oxidation, Carbon sources, Copolyester, Economical production, Genes, Genome, Haloferax mediterranei, Halophilic archaea, PHA biosynthesis, PHA depolymerase, PHA granule, PHA mobilization, PHA synthase, Polyhydroxyalkanoates (PHA), Poly(3-hydroxybutyrate-co- 3-hydroxyvalerate) (PHBHV), Propionyl-CoA, Regulation, Tailor-made PHA, Terpolyester.

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      • 13. app/Models/UserAccess/UserAccess.php:1266
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      • 15. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 16. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
      • 17. vendor/laravel/framework/src/Illuminate/Routing/Route.php:260
    • (select j.user_access_id,j.volume_id,j.user_id,j.from_ip,j.to_ip,j.type,j.track,"Trial Access" as access_base_text, CASE j.type WHEN 111 THEN true ELSE false END as terms_popup, "S" as content_download_type, null as restricted_user_access_key, null as restricted_user_access_type, CASE j.track WHEN 0 THEN false ELSE true END as tracksAccessByVolume,"chapter" as access_level, j.chapter_id from `user_access_chapter_trail` as `j` inner join `user_access_info` as `info` on `j`.`user_access_id` = `info`.`user_access_id` where ((j.from_ip <= 316511805 and j.to_ip >=316511805 )) and ((j.from_date <= 1740514290 and j.to_date >= 1740514290 and j.perpetual= 0) or (j.from_date is null and j.to_date is null and j.perpetual= 1)) and j.chapter_id = 9362) union (select j.user_access_id,j.volume_id,j.user_id,j.from_ip,j.to_ip,j.type,j.track,"Subscribed" as access_base_text, false as terms_popup, "S" as content_download_type, null as restricted_user_access_key, null as restricted_user_access_type, CASE j.track WHEN 0 THEN false ELSE true END as tracksAccessByVolume,"chapter" as access_level, j.chapter_id from `user_access_chapter_corporate` as `j` inner join `user_access_info` as `info` on `j`.`user_access_id` = `info`.`user_access_id` where ((j.from_ip <= 316511805 and j.to_ip >=316511805 )) and ((j.from_date <= 1740514290 and j.to_date >= 1740514290 and j.perpetual= 0) or (j.from_date is null and j.to_date is null and j.perpetual= 1)) and j.chapter_id = 9362) union (select j.user_access_id,j.volume_id,j.user_id,j.from_ip,j.to_ip,j.type,j.track,"Token Based Access" as access_base_text, false as terms_popup, "R" as content_download_type, j.uc_id as restricted_user_access_key, "v" as restricted_user_access_type, CASE j.track WHEN 0 THEN false ELSE true END as tracksAccessByVolume,"chapter" as access_level, j.chapter_id from `user_access_chapter_token` as `j` inner join `user_access_info` as `info` on `j`.`user_access_id` = `info`.`user_access_id` where ((j.from_ip <= 316511805 and j.to_ip >=316511805 )) and ((j.from_date <= 1740514290 and j.to_date >= 1740514290 and j.perpetual= 0) or (j.from_date is null and j.to_date is null and j.perpetual= 1)) and j.chapter_id = 9362)
      700μsalphaeurekaselec_live_10_06_2022UserAccess.php#1266
      Backtrace
      • 13. app/Models/UserAccess/UserAccess.php:1266
      • 14. app/Http/Controllers/ChapterController.php:1549
      • 15. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 16. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
      • 17. vendor/laravel/framework/src/Illuminate/Routing/Route.php:260
    • select b.banner_link, b.banner_name, b.banner_script, b.banner_remarks, b.banner_file_name, b.banner_placement, bd.content_type, bd.content_id from `tbl_banner` as `b` inner join `tbl_banner_detail` as `bd` on `bd`.`banner_id` = `b`.`banner_id` inner join `users` as `u` on `u`.`id` = `b`.`updated_by` where `b`.`banner_ebook` = 1 and `bd`.`content_type` = 'B' and `bd`.`content_id` = 2006 and `b`.`banner_from_date` <= 1740514290 and `b`.`banner_to_date` >= 1740514290 and `b`.`banner_status` = 'A' order by `b`.`banner_id` desc
      1.33msalphaeurekaselec_live_10_06_2022Banner.php#95
      Bindings
      • 0: 1
      • 1: B
      • 2: 2006
      • 3: 1740514290
      • 4: 1740514290
      • 5: A
      Backtrace
      • 13. app/Models/Banner.php:95
      • 14. app/Http/helpers.php:404
      • 17. vendor/laravel/framework/src/Illuminate/Filesystem/Filesystem.php:124
      • 18. vendor/laravel/framework/src/Illuminate/View/Engines/PhpEngine.php:58
      • 19. vendor/laravel/framework/src/Illuminate/View/Engines/CompilerEngine.php:73
    • select count(*) as aggregate from `uc_cart_products` where cart_id='e8b5d0a6eea59e2eef785eb17fde149b'
      230μsalphaeurekaselec_live_10_06_2022Cart.php#271
      Backtrace
      • 15. app/Models/Cart.php:271
      • 16. view::layouts._header:260
      • 18. vendor/laravel/framework/src/Illuminate/Filesystem/Filesystem.php:124
      • 19. vendor/laravel/framework/src/Illuminate/View/Engines/PhpEngine.php:58
      • 20. vendor/laravel/framework/src/Illuminate/View/Engines/CompilerEngine.php:73
    App\Models\Keywords
    22Keywords.php#?
    App\Models\Chapter
    1Chapter.php#?
    App\Models\Ebook
    1Ebook.php#?
        _token
        ghZaDjk24Xehpc6dxIQyKRUMR0W7WnAv5RwUMB7i
        uc_cart_id
        e8b5d0a6eea59e2eef785eb17fde149b
        _previous
        array:1 [ "url" => "https://alpha.eurekaselect.com/chapter/9362" ]
        _flash
        array:2 [ "old" => [] "new" => [] ]
        PHPDEBUGBAR_STACK_DATA
        []
        path_info
        /chapter/9362
        status_code
        200
        
        status_text
        OK
        format
        html
        content_type
        text/html; charset=UTF-8
        request_query
        []
        
        request_request
        []
        
        request_headers
        0 of 0
        array:16 [ "x-https" => array:1 [ 0 => "1" ] "host" => array:1 [ 0 => "alpha.eurekaselect.com" ] "priority" => array:1 [ 0 => "u=0, i" ] "accept-encoding" => array:1 [ 0 => "gzip, deflate, br, zstd" ] "sec-fetch-dest" => array:1 [ 0 => "document" ] "sec-fetch-user" => array:1 [ 0 => "?1" ] "sec-fetch-mode" => array:1 [ 0 => "navigate" ] "sec-fetch-site" => array:1 [ 0 => "none" ] "accept" => array:1 [ 0 => "text/html,application/xhtml+xml,application/xml;q=0.9,image/avif,image/webp,image/apng,*/*;q=0.8,application/signed-exchange;v=b3;q=0.7" ] "user-agent" => array:1 [ 0 => "Mozilla/5.0 AppleWebKit/537.36 (KHTML, like Gecko; compatible; ClaudeBot/1.0; +claudebot@anthropic.com)" ] "upgrade-insecure-requests" => array:1 [ 0 => "1" ] "sec-ch-ua-platform" => array:1 [ 0 => ""Windows"" ] "sec-ch-ua-mobile" => array:1 [ 0 => "?0" ] "sec-ch-ua" => array:1 [ 0 => ""HeadlessChrome";v="129", "Not=A?Brand";v="8", "Chromium";v="129"" ] "cache-control" => array:1 [ 0 => "no-cache" ] "pragma" => array:1 [ 0 => "no-cache" ] ]
        request_cookies
        []
        
        response_headers
        0 of 0
        array:5 [ "content-type" => array:1 [ 0 => "text/html; charset=UTF-8" ] "cache-control" => array:1 [ 0 => "no-cache, private" ] "date" => array:1 [ 0 => "Tue, 25 Feb 2025 20:11:30 GMT" ] "set-cookie" => array:2 [ 0 => "XSRF-TOKEN=eyJpdiI6Inh5T1hEMTJlTEQ4ZEVwTHVLS0RWcXc9PSIsInZhbHVlIjoiM3lWQWxxaXB4K1VrK0x3d3U0enRGQlJOVnF6aFRiZFBONUFHQmtqUjllZURsaEczUDR0b04zMTRoUWZGb29Qc3BFTDhFL3FXNGpaZ0pJYXN5WlZPbVBQaW5wOGthUUxXL2xKMk9pZ3phOFRGMERPcVlwRldEeXIyK0kwcis2aUoiLCJtYWMiOiIwODlhZjNiMDUwZjljMWQ1NTNkNTNhY2Y1MmEyMzJmMDlmN2M0MGY1ODEwMjMzZTg5MzgxNDFlNGU2ZDUyNzQxIiwidGFnIjoiIn0%3D; expires=Fri, 28 Feb 2025 08:11:30 GMT; Max-Age=216000; path=/; secure; samesite=laxXSRF-TOKEN=eyJpdiI6Inh5T1hEMTJlTEQ4ZEVwTHVLS0RWcXc9PSIsInZhbHVlIjoiM3lWQWxxaXB4K1VrK0x3d3U0enRGQlJOVnF6aFRiZFBONUFHQmtqUjllZURsaEczUDR0b04zMTRoUWZGb29Qc3BFTDhFL" 1 => "alphaeurekaselectnet_session=eyJpdiI6IjVlY2dNOXhxNTMvVlVlREZlYkpvR3c9PSIsInZhbHVlIjoic3Z4WG5xNER1bVJoeU8xc3Y0MldoOXdubVZrM25UaWZDY2dUZmt0WnVCU0J3UkRUN0k3RmRkK0tUMXNpYWlyQkFHQ3dMOHBSOVBoQzlMS3I2SVNyODZzK1lBdE9EZDk0RFdzSmUyUjAvMXVFaHlmcnJsSWticGppdzdCOGhkZFEiLCJtYWMiOiI3NGI5MzU0MDIwYjBhNmJmODE4MzFiNmJjZmIzYTQ3NTU3MjNkYTdlMDJkMGZjNjkxZTM4N2QzYjU5MThkNmJkIiwidGFnIjoiIn0%3D; path=/; secure; httponly; samesite=laxalphaeurekaselectnet_session=eyJpdiI6IjVlY2dNOXhxNTMvVlVlREZlYkpvR3c9PSIsInZhbHVlIjoic3Z4WG5xNER1bVJoeU8xc3Y0MldoOXdubVZrM25UaWZDY2dUZmt0WnVCU0J3UkRUN0k3RmRkK0t" ] "Set-Cookie" => array:2 [ 0 => "XSRF-TOKEN=eyJpdiI6Inh5T1hEMTJlTEQ4ZEVwTHVLS0RWcXc9PSIsInZhbHVlIjoiM3lWQWxxaXB4K1VrK0x3d3U0enRGQlJOVnF6aFRiZFBONUFHQmtqUjllZURsaEczUDR0b04zMTRoUWZGb29Qc3BFTDhFL3FXNGpaZ0pJYXN5WlZPbVBQaW5wOGthUUxXL2xKMk9pZ3phOFRGMERPcVlwRldEeXIyK0kwcis2aUoiLCJtYWMiOiIwODlhZjNiMDUwZjljMWQ1NTNkNTNhY2Y1MmEyMzJmMDlmN2M0MGY1ODEwMjMzZTg5MzgxNDFlNGU2ZDUyNzQxIiwidGFnIjoiIn0%3D; expires=Fri, 28-Feb-2025 08:11:30 GMT; path=/; secureXSRF-TOKEN=eyJpdiI6Inh5T1hEMTJlTEQ4ZEVwTHVLS0RWcXc9PSIsInZhbHVlIjoiM3lWQWxxaXB4K1VrK0x3d3U0enRGQlJOVnF6aFRiZFBONUFHQmtqUjllZURsaEczUDR0b04zMTRoUWZGb29Qc3BFTDhFL" 1 => "alphaeurekaselectnet_session=eyJpdiI6IjVlY2dNOXhxNTMvVlVlREZlYkpvR3c9PSIsInZhbHVlIjoic3Z4WG5xNER1bVJoeU8xc3Y0MldoOXdubVZrM25UaWZDY2dUZmt0WnVCU0J3UkRUN0k3RmRkK0tUMXNpYWlyQkFHQ3dMOHBSOVBoQzlMS3I2SVNyODZzK1lBdE9EZDk0RFdzSmUyUjAvMXVFaHlmcnJsSWticGppdzdCOGhkZFEiLCJtYWMiOiI3NGI5MzU0MDIwYjBhNmJmODE4MzFiNmJjZmIzYTQ3NTU3MjNkYTdlMDJkMGZjNjkxZTM4N2QzYjU5MThkNmJkIiwidGFnIjoiIn0%3D; path=/; secure; httponlyalphaeurekaselectnet_session=eyJpdiI6IjVlY2dNOXhxNTMvVlVlREZlYkpvR3c9PSIsInZhbHVlIjoic3Z4WG5xNER1bVJoeU8xc3Y0MldoOXdubVZrM25UaWZDY2dUZmt0WnVCU0J3UkRUN0k3RmRkK0t" ] ]
        session_attributes
        0 of 0
        array:5 [ "_token" => "ghZaDjk24Xehpc6dxIQyKRUMR0W7WnAv5RwUMB7i" "uc_cart_id" => "e8b5d0a6eea59e2eef785eb17fde149b" "_previous" => array:1 [ "url" => "https://alpha.eurekaselect.com/chapter/9362" ] "_flash" => array:2 [ "old" => [] "new" => [] ] "PHPDEBUGBAR_STACK_DATA" => [] ]
        ClearShow all
        Date ↕MethodURLData
        #12025-02-25 20:11:30GET/chapter/93621121924