Abstract
Information regarding the distance between paired reads enhances the accuracy of genome assembly and sequence-to-genome mapping, making paired-end indispensable strategies for DNA sequencing. The most commonly used paired-end sequencing strategies are Paired-End (PE) sequencing and Paired-End Ditag (PED) sequencing. Similarity in terminologies frequently causes confusion. This chapter is set out to clarify these terminologies and then, using PED as an example, to illustrate how a biotechnology can be sequentially developed.
Keywords: bPED, ChIP-EM, ChIP-HM, ChIP-TFBS, in situ PCR, mbPED, PE sequencing, PED sequencing.
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